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Goat IgG ELISA Kit

Product name

Immunoglobulin G (IgG), ELISA Kit

Full product name

Goat Immunoglobulin G ELISA Kit

Product gene name

IgG Elisa kit

For research use only

For research use only. It should not be used in diagnostic procedures.

Species reactivity: Goat

Specificity

This assay has high sensitivity and excellent specificity for the detection of IgG. No significant cross-reactivity or interference between IgG and analogues was observed.

Samples

Serum, plasma, tissue homogenates and other biological fluids

Test type: Quantitative sandwich

Detection range: 1,563-100ng/ml

Sensitivity: 0.938ng/mL

Intra-assay precision: CV<8%

Inter-assay precision: CV<10%

Preparation and Storage

Store at 4 degrees C if the kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, lyophilized standard, and concentrated biotinylated detection protein are stored at -20 degrees C. Other components at 2-8 degrees C). Stable for 12 months (if the entire kit is stored at -20 degrees C).

ISO certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 certified laboratory.

Goat ELISA Kits | High Quality Research Tools

Product note

Information selected from the online data sheet is extracted from bioinformatics databases, sometimes resulting in the ambiguous or non-relevant product information. It is the customer’s responsibility to review, verify and evaluate the information to ensure it matches their requirements prior to purchasing the kit. Our ELISA Kit assays are dynamic research tools and may be updated and improved from time to time.

If the format of this assay is important to you, please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current trial details. We cannot guarantee that the sample manual posted online is the most up-to-date manual, it is intended to serve as an example only. Consult the instructions for use provided with the assay kit for precise details.

Other notes

Occasionally, small volumes of Goat IgG Elisa kit vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly spin the vial in a tabletop centrifuge to dislodge any liquid in the vial cap. Certain products may require dry ice shipping and an additional dry ice fee may apply.

Search terms for the purchase of IgG

MBS765028 is a ready-to-use microwell and strip plate ELISA (Enzyme-Linked Immunosorbent Assay) Kit for testing the presence of Immunoglobulin G (IgG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls generate a theoretical detection range of the kit in biological research samples containing IgG.

The analytical biochemical ELISA technique of the MBS765028 kit is based on IgG antibody-IgG antigen interactions (immunoabsorption) and an HRP colourimetric detection system to detect IgG antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, and secretions. Quality control assays that evaluated reproducibility identified intra-assay CV (%) and inter-assay CV (%).

Product related information for IgG Elisa kit

Assay Principle: This kit was based on sandwich enzyme-linked immunosorbent assay technology. The capture antibody was pre-coated onto 96-well plates. And biotin-conjugated antibody was used as detection antibodies. Standards, test samples, and biotin-conjugated detection antibodies were subsequently added to the wells and washed with wash buffer. HRP-streptavidin was added and unbound conjugates were washed with wash buffer.

TMB substrates were used to visualize the enzymatic reaction of HRP. TMB was catalyzed by HRP to produce a blue product that changed to yellow after adding an acid stop solution. The density of yellow is proportional to the target amount of sample captured on the plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the target concentration can be calculated.

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