BioConstructor Molecular Cloning Software

Lenti vector viral particles from E. coli plasmids with Puro

Total Goat Milk Proteins ELISA Kit

Description The Total Goat Milk Proteins ELISA kit is designed to determine the presence of goat milk protein impurities in products manufactured by transgenic expression of goat milk. This kit has a LOD of ~0.1 ng/mL. Use Sample Diluent, item #F243A, with these kits; available separately. Product Information Product Type: ELISA Kit Storage: Standards -10°C to -30°C upon receipt, All other kit reagents 2°C to 8°C Assay format: 96-well plate...

Goat IgG ELISA Kit

Product name Immunoglobulin G (IgG), ELISA Kit Full product name Goat Immunoglobulin G ELISA Kit Product gene name IgG Elisa kit For research use only For research use only. It should not be used in diagnostic procedures. Species reactivity: Goat Specificity This assay has high sensitivity and excellent specificity for the detection of IgG. No significant cross-reactivity or interference between IgG and analogues was observed. Samples Serum,...

Ultra-Sensitive Insulin ELISA Kit

Specifications Catalogue #: 80-INSHUU-E01.1 Species: Human Regulatory status: FDA class I devise. For in vitro diagnostic use. Product distribution: Available worldwide Distance: 0.15 - 20 µIU/mL Sensitivity: 0.135 µIU/mL Sizes: 96 wells Sample types: plasma, serum Incubation time: 1 hour 30 minutes Sample size: 25μL Detection: colorimetric Additional Information The Ultrasensitive Insulin ELISA Kit is an FDA-registered in vitro diagnostic...

Assessment of Bacillus subtilis Plasmid pLS20 Conjugation in the Absence of Quorum Sensing Repression

Bacillus subtilis conjugative plasmid pLS20 makes use of a quorum-sensing mechanism to regulate expression ranges of its conjugation genes, involving the repressor RcopLS20, the anti-repressor RappLS20, and the signaling peptide Phr*pLS20. In earlier research, synthetic overexpression of rappLS20 within the donor cells was proven to boost conjugation effectivity. Nonetheless, we discovered that the overexpression of rappLS20 led to varied...

Acquisition of the Conjugative Virulence Plasmid From a CG23 Hypervirulent Klebsiella pneumoniae Strain Enhances Bacterial Virulence

The emergence of hypervirulent and carbapenem-resistant Klebsiella pneumoniae (hv-CRKP) has turn into a scorching subject and confounding drawback for clinicians and researchers alike. Conjugative virulence plasmids have the potential to trigger extra threatening dissemination of hv-CRKP strains. We beforehand recognized Ok2606, a CG23 scientific hypervirulent pressure of Klebsiella pneumoniae harboring a conjugative virulence plasmid...

Development of an entirely plasmid-based reverse genetics system for 12-segmented double-stranded RNA viruses

The household Reoviridae is a nonenveloped virus group with a double-stranded (ds) RNA genome comprising 9 to 12 segments. Within the household Reoviridae, the genera Cardoreovirus, Phytoreovirus, Seadornavirus, Mycoreovirus, and Coltivirus comprise virus species having 12-segmented dsRNA genomes. Reverse genetics programs used to generate recombinant infectious viruses are highly effective instruments for investigating viral gene...

The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in E. coli

We describe a brand new set of instruments for inserting DNA into the bacterial chromosome. The system makes use of site-specific recombination reactions carried out by bacteriophage integrases to combine plasmids at as much as eight phage attachment websites in E. coli MG1655. The introduction of mutant loxP websites within the integrating plasmids permits repeated elimination of antibiotic resistance genes and different plasmid sequences...