BioConstructor Molecular Cloning Software

Lenti vector viral particles from E. coli plasmids with Puro

Acquisition of the Conjugative Virulence Plasmid From a CG23 Hypervirulent Klebsiella pneumoniae Strain Enhances Bacterial Virulence

The emergence of hypervirulent and carbapenem-resistant Klebsiella pneumoniae (hv-CRKP) has turn into a scorching subject and confounding drawback for clinicians and researchers alike. Conjugative virulence plasmids have the potential to trigger extra threatening dissemination of hv-CRKP strains. We beforehand recognized Ok2606, a CG23 scientific hypervirulent pressure of Klebsiella pneumoniae harboring a conjugative virulence plasmid...

Development of an entirely plasmid-based reverse genetics system for 12-segmented double-stranded RNA viruses

The household Reoviridae is a nonenveloped virus group with a double-stranded (ds) RNA genome comprising 9 to 12 segments. Within the household Reoviridae, the genera Cardoreovirus, Phytoreovirus, Seadornavirus, Mycoreovirus, and Coltivirus comprise virus species having 12-segmented dsRNA genomes. Reverse genetics programs used to generate recombinant infectious viruses are highly effective instruments for investigating viral gene...