BioConstructor Molecular Cloning Software

Lenti vector viral particles from E. coli plasmids with Puro

Development of an entirely plasmid-based reverse genetics system for 12-segmented double-stranded RNA viruses

The household Reoviridae is a nonenveloped virus group with a double-stranded (ds) RNA genome comprising 9 to 12 segments. Within the household Reoviridae, the genera Cardoreovirus, Phytoreovirus, Seadornavirus, Mycoreovirus, and Coltivirus comprise virus species having 12-segmented dsRNA genomes. Reverse genetics programs used to generate recombinant infectious viruses are highly effective instruments for investigating viral gene...