Laboratory Exercise to Measure Plasmid Copy Number by qPCR
Quantitative PCR (qPCR) has quite a few purposes in biology. In an academic setting, qPCR supplies college students a possibility to higher perceive the PCR mechanism by offering each quantitative details about the reactions and likewise knowledge to troubleshoot PCRs (e.g., soften curves). Right here, we current a comparatively brief (2-h) laboratory exercise to display qPCR to quantify plasmid copy quantity (CN) by measuring the cycle...