A two-plasmid system for identification of promoters recognized by RNA polymerase containing extracytoplasmic stress response sigma(E) in Escherichia coli.
We have now beforehand established a two-plasmid system in Escherichia coli for identification of promoters acknowledged by RNA polymerase containing a heterologous sigma issue. Makes an attempt to optimize this method for identification of promoters acknowledged by RNA polymerase containing E. coli extracytoplasmic stress response sigma(E) failed owing to excessive toxicity of the expressed rpoE. A brand new system for identification of...