BioConstructor Molecular Cloning Software

Lenti vector viral particles from E. coli plasmids with Puro

Structural and functional analysis of the kid toxin protein from E. coli plasmid R1.

Structural and functional analysis of the kid toxin protein from E. coli plasmid R1.

We have now decided the construction of Child toxin protein from E. coli plasmid R1 concerned in steady plasmid inheritance by postsegregational killing of plasmid-less daughter cells. Child varieties a two-component system with its antagonist, Kis antitoxin. Our 1.four A crystal construction of Child reveals a 2-fold symmetric dimer that intently resembles the DNA gyrase-inhibitory toxin protein CcdB from E. coli F plasmid regardless of the shortage of any notable sequence similarity. Evaluation of unhazardous mutants of Child suggests a goal interplay interface related to toxicity that’s in marked distinction to that proposed for CcdB. A potential area for interplay of Child with the antitoxin is proposed that overlaps with the goal binding web site and will clarify the mode of antitoxin motion.

The expression product of CPZr was beta-lactamase. The excessive stage beta-lactamase enzymatic actions towards cephaloridine of CPZr transformants which had been detected spectrophotometrically at 260 nm wave size demonstrated excessive stage similarities to that of pFC. MICs of 18 antibiotics had been decided in accordance with a tenet of NCCLS by broth dilution methodology. CPZr transformants confirmed reasonable stage resistance to ampicillin, cefazolin, cefazolin, cefamandole and CPZ (MIC, 64 micrograms/ml).

In the meantime, susceptibility testing outcomes demonstrated that the extent of resistance to CPZ of pFC transformant on this examine (MIC, 64 micrograms/ml) was a lot decrease than that in 1988 (MIC,>> 512 micrograms/ml) and resistance to nofloxacin and aminoglycosides was not noticed. Induction experiment and temperature-sensitive mutation of CPZ resistance had been carried out. CPZr colonal strains revealed the higher-level of resistance to CPZ (MIC, 512 micrograms/ml) attributable to antibiotic CPZ induction moderately than temperature delicate mutation.

[Coexpression of caiB and caiE with two plasmids in Ecoli].

Recombinant micro organism exhibiting excessive enzymatic actions had been obtained by cloning and coexpression of each caiB gene and caiE gene within the host of E. coli Bl21(DE3), which encode carnitine dehydratase and a protein associated to the synthesis of cofactor for carnitine dehydratase, respectively. In an effort to coexpress these two genes, suitable and incompatible two plasmids system had been used, the distinction between them was additionally studied. After induction with IPTG, each caiB and caiE genes had been coexpressed in each suitable two plasmids system and incompatible two plasmids system. Within the former system, the expressed merchandise accounted for 17% and 10% of the full proteins within the host; within the later system, the proportion was 39% and 20%, respectively.

Structural and functional analysis of the kid toxin protein from E. coli plasmid R1.

The exercise of carnitine dehydratase in E. coli Bl21(DE3) with both coexpression system is about 2.three instances than that in E. coli Bl21(DE3) with solely pET28-caiB. The plasmids stabilities in these two methods had been the identical, all wanted the assistance of antibiotic selective strain. A handy and fast methodology for the genetic transformation of Escherichia coli with plasmids is proposed. By mixing the recipient cells and plasmid DNA and spreading them immediately on selective medium plates containing Ca2+, the so-called ‘plate transformation’ may obtain virtually the identical transformation effectivity because the classical transformation methodology with calcium.

The entire protocol takes solely about 2 min, its simplicity in contrast favorably, not solely to the standard protocol, but additionally to all different documented modifications. E. coli ATCC-27166, the pressure most delicate to all medication on this experiment, was employed for disc diffusion experiments and from the sample of look of the inhibition zone, eight main antibiotics had been divided into three teams relying on their exercise towards containing every of the three plasmids. Solely gentamicin (GM) exercise was not affected by any of the drug resistant strains. Assay strategies using three resistant strains could be the approach for screening meals for antibiotic residues sooner or later.

proBA complementation of an auxotrophic Ecoli pressure improves plasmid stability and expression yield throughout fermenter manufacturing of a recombinant antibody fragment.

The proline-auxotrophic Escherichia coli Ok12 pressure JM83 harbouring an expression vector offering the proBA gene in trans was utilized for the fermenter manufacturing of the partially humanized IN-1 antibody F(ab) fragment. Thus, plasmid-mediated complementation of the chromosomal proBA deletion was employed as a second choice mechanism, along with a chloramphenicol resistance, with the intention to (i) abolish plasmid loss and (ii) profit from E. coli JM83 as an expression pressure with accepted periplasmic protein secretion traits within the presence of a minimal medium.

Ranging from the generic vector pASK75, which makes use of the tightly regulated and chemically inducible tet promoter for overseas gene expression, a set of latest vectors carrying all the or a part of the proBA operon was constructed and in contrast regarding their functionality of practical Delta proBA complementation in addition to recombinant protein yield. Because of this, the vector pMF1 was developed, the place transcription of the proBA operon is managed by its personal constitutive promoter and terminator sequences, allowing the reworked JM83 pressure to develop below glucose/ammonia minimal tradition situations. When pMF1 was used for the fermenter manufacturing of the IN-1 F(ab) fragment, no plasmid loss was noticed through the development and induction phases, and the yield of functionally purified recombinant protein was discovered to be significantly improved.

Escherichia coli (E. coli) Antibody

abx415712-1ml 1 ml
EUR 606

E. coli Topoisomerase IV Drug Screening Kit (62.5ug SC DNA)

TG1007-2 250 assays
EUR 448

E. coli GroL

DAG1704 100 ug
EUR 915

E. coli GroS

DAG1705 100 ug
EUR 915

E. coli DsbA

DAG1712 100 ug
EUR 915

E. coli GrpE

DAG1714 100 ug
EUR 1027

E. coli Antibody

abx021669-1mg 1 mg
EUR 1295

E. coli Antibody

abx021670-1mg 1 mg
EUR 871

E. coli Antibody

abx021671-02ml 0.2 ml
EUR 495

E. coli Antibody

abx021672-1mg 1 mg
EUR 871

E. coli Antibody

abx021673-1mg 1 mg
EUR 871

E. coli Antibody

abx022970-1ml 1 ml
EUR 732

E. coli Antibody

abx022971-1ml 1 ml
EUR 968

E. coli Antibody

abx022975-1ml 1 ml
EUR 599

E. coli Antibody

abx022977-1ml 1 ml
EUR 676

E. coli antibody

20-ER13 1 ml
EUR 355
Description: Rabbit polyclonal E. coli antibody

E. coli antibody

20C-CR1244GP 1 ml
EUR 468
Description: Goat polyclonal E. coli antibody

E. coli antibody

10-E09C 100 ug
EUR 133
Description: Mouse monoclonal E. coli antibody

E. coli antibody

10-E10A 500 ug
EUR 493
Description: Mouse monoclonal E. coli antibody

E. coli antibody

10-E10B 500 ug
EUR 493
Description: Mouse monoclonal E. coli antibody

E. coli antibody

10C-CR1244M1 100 ug
EUR 408
Description: Mouse monoclonal E. coli antibody

E. coli (SLT-2, STX-2) Antibody

abx021674-1mg 1 mg
EUR 1428

BMP-2 (E. coli-expressed), CF

PR15014CF 10 ug
EUR 311

E. coli STX 1 & 2 Antibody

abx021692-1mg 1 mg
EUR 1344

Inactivated DENV-2 Antigen (E. coli)

VAng-Lsx0084-1mg 1 mg
EUR 822
Description: DENV-2, natural antigen from E. coli.

Escherichia coli (E. coli) Antibody (Biotin)

abx411321-1ml 1 ml
EUR 592

Escherichia coli (E. coli) Antibody (FITC)

abx411322-1ml 1 ml
EUR 509

Escherichia coli (E. coli) Antibody (Biotin)

abx415713-1ml 1 ml
EUR 718

Recombinant HIV-2 E gp 36 Protein (E. coli)

VAng-0534Lsx-inquire inquire Ask for price
Description: HIV type 2 Envelope glycoprotein 36, recombinant protein from E. coli. MW 16 kDa, 1 mg/mL.

Recombinant (E. coli) Dengue Virus Type 2 (and epitopes for type 1, 2, and 3) E Antigen (DENV E) (23 kda, >95% pure)

RP-1598 50 ug
EUR 408

E. coli FimC [His]

DAG1666 100 ug
EUR 1027

E. coli Eco [His]

DAG1667 50 ug
EUR 1027

E. coli RecA Protein

01-001 200 ug
EUR 205
Description: The E. coli RecA Protein is available in Europe and for worldwide shipping via Gentaur.

E. coli LexA repressor

01-005 50 ug
EUR 479
Description: The E. coli LexA repressor is available in Europe and for worldwide shipping via Gentaur.

E. coli LexA repressor

01-006 250 ug
EUR 1184
Description: The E. coli LexA repressor is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvA protein

01-007 50 ug
EUR 479
Description: The E. coli RuvA protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvA protein

01-008 250 ug
EUR 1184
Description: The E. coli RuvA protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvB protein

01-009 50 ug
EUR 479
Description: The E. coli RuvB protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvB protein

01-010 250 ug
EUR 1184
Description: The E. coli RuvB protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvC protein

01-011 50 ug
EUR 479
Description: The E. coli RuvC protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvC protein

01-012 250 ug
EUR 1184
Description: The E. coli RuvC protein is available in Europe and for worldwide shipping via Gentaur.

E. coli GroEL Antibody

20012-05011 150 ug
EUR 217

E. coli CysH Antibody

30281-05111 150 ug
EUR 261

E. coli DnaJ Antibody

32022-05111 150 ug
EUR 261

E. coli pykF Antibody

32163-05111 150 ug
EUR 261

E. coli GOR Antibody

32540-05111 150 ug
EUR 261

E. coli NDK Antibody

32662-05111 150 ug
EUR 261

E. coli PPA Antibody

32678-05111 150 ug
EUR 261

E. coli glk Antibody

32864-05111 150 ug
EUR 261

E. Coli ldhA Antibody

32898-05111 150 ug
EUR 261

E. coli deoC Antibody

32936-05111 150 ug
EUR 261

E. coli Skp Antibody

32971-05111 150 ug
EUR 261

E. coli nanA Antibody

33092-05111 150 ug
EUR 261

E. coli O157 Antibody

abx024017-1mg 1 mg
EUR 1010

E. coli O157 Antibody

abx021684-1mg 1 mg
EUR 1017

E. coli O157 Antibody

abx021685-1mg 1 mg
EUR 996

E. coli O157 Antibody

abx021686-1mg 1 mg
EUR 996

E. coli O157 Antibody

abx021687-1mg 1 mg
EUR 996

E. coli O157 Antibody

abx021688-1mg 1 mg
EUR 940

E. coli O157 Antibody

abx021689-1mg 1 mg
EUR 996

E. coli Antibody (HRP)

abx022972-1ml 1 ml
EUR 599

E. coli Antibody (FITC)

abx022974-1ml 1 ml
EUR 648

E. coli Antibody (Biotin)

abx022976-1ml 1 ml
EUR 599

E. coli Antibody (HRP)

abx022978-1ml 1 ml
EUR 878

E. coli Antibody (Biotin)

abx022979-1ml 1 ml
EUR 739

E. coli O 157

abx291002-100mg 100 mg
EUR 620

E. coli Topoisomerase IV

TG2000EC-1 250 units
EUR 448

E. coli Topoisomerase IV

TG2000EC-3 500 units
EUR 683

E. coli Topoisomerase IV

TG2000EC-5 1000 units
EUR 1052

E. coli DNA Gyrase

TG2000G-1 100 units
EUR 324

E. coli DNA Gyrase

TG2000G-3 500 units
EUR 705

E. coli DNA Gyrase

TG2000G-5 1000 units
EUR 1164

E. coli DNA Gyrase

TG2000G-7 2000 units
EUR 1836

Enterotoxin STp (E. coli)

H-6248.0500 0.5mg
EUR 696
Description: Sum Formula: C81H110N20O26S6; CAS# [115474-04-9] net

Enterotoxin STp (E. coli)

H-6248.1000 1.0mg
EUR 1180
Description: Sum Formula: C81H110N20O26S6; CAS# [115474-04-9] net

E. coli antibody (FITC)

60-E13 1 ml
EUR 378
Description: Rabbit polyclonal E. coli antibody (FITC) conjugated

E. coli antibody (biotin)

60-E13B 1 ml
EUR 468
Description: Rabbit polyclonal E. coli antibody (biotin) conjugated

E. coli antibody (HRP)

60-E14 1 ml
EUR 408
Description: Rabbit polyclonal E. coli antibody (HRP) conjugated

E. coli O157 antibody

10-1490 100 ug
EUR 136
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody

10-1521 100 ug
EUR 142
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody

10-E12A 100 ug
EUR 385
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody

10C-CR1295M3 100 ug
EUR 392
Description: Mouse monoclonal E. coli O157 antibody

E. Coli HCP antibody

20R-EG001 1 ml
EUR 837
Description: Goat polyclonal E. Coli HCP antibody

E. coli DNA Ligase

M1217-100
EUR 321

E. coli Exonuclease I

M1222-100
EUR 251

E. coli Exonuclease III

M1223-100
EUR 267

RecA Protein E. coli

M1231-200
EUR 202

E. coli Recombinant IDNK

P1050-10
EUR 164

E. coli Recombinant IDNK

P1050-50
EUR 381

Recombinant E. coli, Glucokinase

P1054-10
EUR 164

Recombinant E. coli, Glucokinase

P1054-50
EUR 381

E. Coli Recombinant TRXB

6331-100
EUR 425

E. Coli Recombinant SlyD

6366-100
EUR 457

Benzonase, E. Coli Recombinant

7680-25
EUR 332

Benzonase, E. Coli Recombinant

7680-5
EUR 137

TesA, E. coli esterase

0306-1 100 Units
EUR 421

TesA, E. coli esterase

0306-5 500 Units
EUR 1705

Recombinant (E. coli) Human Tumour Type M2 Pyruvate Kinase

RP-744 10 ug
EUR 286

Human Escherichia coli (E. coli) ELISA Kit

abx052493-96tests 96 tests
EUR 668

Cow Escherichia coli (E. coli) ELISA Kit

abx055785-96tests 96 tests
EUR 668

E. coli Shiga toxin 2 PCR kit

PCR-VH108-48D 50T
EUR 453

E. coli Shiga toxin 2 PCR kit

PCR-VH108-96D 100T
EUR 572

E. coli TrxA (aa 2 - 109) [His]

DAG1725 50 ug
EUR 1027

E. coli Carbonic Anhydrase 2 (CA2) Antibody

30109-05111 150 ug
EUR 261

E. coli O157 Verotoxin 2 (VT2) Protein

abx061527-100ug 100 ug
EUR 1010

Recombinant HSV-2 gD Protein (E. coli)

VAng-0548Lsx-inquire inquire Ask for price
Description: The E. coli derived recombinant protein contains the HSV-2 Glycoprotein D (gD) immunodominant regions. MW 40.6 kDa, 1.00 mg/mL.

MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody

20-abx113898
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody

abx034997-400ul 400 ul
EUR 523

MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody

abx034997-80l 80 µl
EUR 286

MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody

20-abx133688
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody

20-abx000543
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul

MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody

20-abx001036
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody

abx011203-100ul 100 ul
EUR 411

MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody

abx026331-400ul 400 ul
EUR 523

MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody

abx026331-80l 80 µl
EUR 286

MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody

abx235371-100ug 100 ug
EUR 481

MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody

abx235372-100ug 100 ug
EUR 551

MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody

abx331633-100ul 100 ul
EUR 425

MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody

20-abx326761
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

ELISA kit for Human RAG-2 (Recombination Activating Gene 2)

E-EL-H2320 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human RAG-2 (Recombination Activating Gene 2) in samples from Serum, Plasma, Cell supernatant

E. Coli Proteins-Agarose affinity gel for removing E. coli antibodies

EC11-G 1 ml
EUR 286

Coelenterazine e

10124-2 250uG
EUR 399
Description: Minimum order quantity: 1 unit of 250uG

Nori® Human Pre-Legumain ELISA Kit (2 plates)

GR106359-2 2 x 96-well
EUR 832

Escherichia coli Serotype O2 (E. coli O2) Antibody

abx411330-02mg 0.2 mg
EUR 565

Escherichia coli Serotype O15 (E. coli O15) Antibody

abx411331-02mg 0.2 mg
EUR 565

Escherichia coli Serotype O20 (E. coli O20) Antibody

abx411332-02mg 0.2 mg
EUR 565

Human pre-microRNA Expression Construct 146a-2

PMIRH146a-PA-2 Bacterial Streak
EUR 684

Recombinant Diacylglycerol Kinase (E. coli)

MET-5035 100 mg
EUR 276
Description: Purified recombinant prokaryotic DAG kinase expressed in E. coli

Recombinant Diacylglycerol Kinase (E. coli)

MET-5035-5 5 x 100 mg
EUR 920
Description: Purified recombinant prokaryotic DAG kinase expressed in E. coli

E. coli β-gal peptide

DAG2441 0.05 mg
EUR 485

Native E. Coli O 158

DAGA-3025 0.1 ml
EUR 715

Uricase from E. coli, Recombinant

DIA-415 5KU
EUR 378

E. coli RecQ DNA helicase

01-003 50 ug
EUR 479
Description: The E. coli RecQ DNA helicase is available in Europe and for worldwide shipping via Gentaur.

E. coli RecQ DNA helicase

01-004 250? g
EUR 1549
Description: The E. coli RecQ DNA helicase is available in Europe and for worldwide shipping via Gentaur.

E. coli Glycerol Kinase Antibody

32699-05111 150 ug
EUR 261

E. coli Tryptophanase (Tnase) Antibody

33517-05111 150 ug
EUR 261

Mouse antibody for E. coli

1063 100 ug
EUR 365.38
Description: This is Mouse monoclonal FITC conjugated antibody against E coli O157 for WB, ELISA.

E. coli O157 (9.88) Antibody

BNC040207-100 100uL
EUR 199
Description: Primary antibody against E. coli O157 (9.88),CF405S conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody

BNC040207-500 500uL
EUR 544
Description: Primary antibody against E. coli O157 (9.88),CF405S conjugate, Concentration: 0.1mg/mL

E. Coli O 157 Protein

abx061475-01ml 0.1 ml
EUR 551

E. coli O157 (9.88) Antibody

BNC470207-100 100uL
EUR 199
Description: Primary antibody against E. coli O157 (9.88),CF647 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody

BNC470207-500 500uL
EUR 544
Description: Primary antibody against E. coli O157 (9.88),CF647 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody

BNC550207-100 100uL
EUR 199
Description: Primary antibody against E. coli O157 (9.88),CF555 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody

BNC550207-500 500uL
EUR 544
Description: Primary antibody against E. coli O157 (9.88),CF555 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody

BNCB0207-100 100uL
EUR 199
Description: Primary antibody against E. coli O157 (9.88),Biotin conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody

BNCB0207-500 500uL
EUR 544
Description: Primary antibody against E. coli O157 (9.88),Biotin conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody

BNC810207-100 100uL
EUR 199
Description: Primary antibody against E. coli O157 (9.88),CF680R conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody

BNC810207-500 500uL
EUR 544
Description: Primary antibody against E. coli O157 (9.88),CF680R conjugate, Concentration: 0.1mg/mL

E. coli Enterotoxin B Antibody

abx021678-1mg 1 mg
EUR 599

E. coli Enterotoxin B Antibody

abx021679-1mg 1 mg
EUR 606

Utilizing three completely different plasmid DNA codings for kanamycin (KM), chloramphenicol (CP), and ampicillin- (AMP) and tetracycline- (TC) resistance, 4 completely different competent Escherichia coli strains had been reworked by the calcium chloride methodology to provide KM-, CP- and AMP- and TC-resistant strains. Analysis of minimal inhibitory concentrations (MIC) of 22 antibiotics, confirmed KM-resistant E. coli to be cross resistant solely to fradiomycin (FRM); CP-resistant E. coli, particularly HB101 and JM109 strains, exhibited cross-resistance solely to thiamphenicol (TP). However, AMP- and TC-resistant E. coli confirmed cross resistance to a number of penicillins, tetracyclines and erythromycin.

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