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Structural and functional analysis of the kid toxin protein from E. coli plasmid R1.

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Structural and functional analysis of the kid toxin protein from E. coli plasmid R1.

We have now decided the construction of Child toxin protein from E. coli plasmid R1 concerned in steady plasmid inheritance by postsegregational killing of plasmid-less daughter cells. Child varieties a two-component system with its antagonist, Kis antitoxin. Our 1.four A crystal construction of Child reveals a 2-fold symmetric dimer that intently resembles the DNA gyrase-inhibitory toxin protein CcdB from E. coli F plasmid regardless of the shortage of any notable sequence similarity. Evaluation of unhazardous mutants of Child suggests a goal interplay interface related to toxicity that’s in marked distinction to that proposed for CcdB. A potential area for interplay of Child with the antitoxin is proposed that overlaps with the goal binding web site and will clarify the mode of antitoxin motion.

The expression product of CPZr was beta-lactamase. The excessive stage beta-lactamase enzymatic actions towards cephaloridine of CPZr transformants which had been detected spectrophotometrically at 260 nm wave size demonstrated excessive stage similarities to that of pFC. MICs of 18 antibiotics had been decided in accordance with a tenet of NCCLS by broth dilution methodology. CPZr transformants confirmed reasonable stage resistance to ampicillin, cefazolin, cefazolin, cefamandole and CPZ (MIC, 64 micrograms/ml).

In the meantime, susceptibility testing outcomes demonstrated that the extent of resistance to CPZ of pFC transformant on this examine (MIC, 64 micrograms/ml) was a lot decrease than that in 1988 (MIC,>> 512 micrograms/ml) and resistance to nofloxacin and aminoglycosides was not noticed. Induction experiment and temperature-sensitive mutation of CPZ resistance had been carried out. CPZr colonal strains revealed the higher-level of resistance to CPZ (MIC, 512 micrograms/ml) attributable to antibiotic CPZ induction moderately than temperature delicate mutation.

[Coexpression of caiB and caiE with two plasmids in E. coli].

Recombinant micro organism exhibiting excessive enzymatic actions had been obtained by cloning and coexpression of each caiB gene and caiE gene within the host of E. coli Bl21(DE3), which encode carnitine dehydratase and a protein associated to the synthesis of cofactor for carnitine dehydratase, respectively. In an effort to coexpress these two genes, suitable and incompatible two plasmids system had been used, the distinction between them was additionally studied. After induction with IPTG, each caiB and caiE genes had been coexpressed in each suitable two plasmids system and incompatible two plasmids system. Within the former system, the expressed merchandise accounted for 17% and 10% of the full proteins within the host; within the later system, the proportion was 39% and 20%, respectively.

Structural and functional analysis of the kid toxin protein from E. coli plasmid R1.

The exercise of carnitine dehydratase in E. coli Bl21(DE3) with both coexpression system is about 2.three instances than that in E. coli Bl21(DE3) with solely pET28-caiB. The plasmids stabilities in these two methods had been the identical, all wanted the assistance of antibiotic selective strain. A handy and fast methodology for the genetic transformation of Escherichia coli with plasmids is proposed. By mixing the recipient cells and plasmid DNA and spreading them immediately on selective medium plates containing Ca2+, the so-called ‘plate transformation’ may obtain virtually the identical transformation effectivity because the classical transformation methodology with calcium.

The entire protocol takes solely about 2 min, its simplicity in contrast favorably, not solely to the standard protocol, but additionally to all different documented modifications. E. coli ATCC-27166, the pressure most delicate to all medication on this experiment, was employed for disc diffusion experiments and from the sample of look of the inhibition zone, eight main antibiotics had been divided into three teams relying on their exercise towards containing every of the three plasmids. Solely gentamicin (GM) exercise was not affected by any of the drug resistant strains. Assay strategies using three resistant strains could be the approach for screening meals for antibiotic residues sooner or later.

proBA complementation of an auxotrophic E. coli pressure improves plasmid stability and expression yield throughout fermenter manufacturing of a recombinant antibody fragment.

The proline-auxotrophic Escherichia coli Ok12 pressure JM83 harbouring an expression vector offering the proBA gene in trans was utilized for the fermenter manufacturing of the partially humanized IN-1 antibody F(ab) fragment. Thus, plasmid-mediated complementation of the chromosomal proBA deletion was employed as a second choice mechanism, along with a chloramphenicol resistance, with the intention to (i) abolish plasmid loss and (ii) profit from E. coli JM83 as an expression pressure with accepted periplasmic protein secretion traits within the presence of a minimal medium.

Ranging from the generic vector pASK75, which makes use of the tightly regulated and chemically inducible tet promoter for overseas gene expression, a set of latest vectors carrying all the or a part of the proBA operon was constructed and in contrast regarding their functionality of practical Delta proBA complementation in addition to recombinant protein yield. Because of this, the vector pMF1 was developed, the place transcription of the proBA operon is managed by its personal constitutive promoter and terminator sequences, allowing the reworked JM83 pressure to develop below glucose/ammonia minimal tradition situations. When pMF1 was used for the fermenter manufacturing of the IN-1 F(ab) fragment, no plasmid loss was noticed through the development and induction phases, and the yield of functionally purified recombinant protein was discovered to be significantly improved.

Escherichia coli (E. coli) Antibody
abx415692-01mg 0.1 mg
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E. coli antibody
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E. coli antibody
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E. coli GroL
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E. coli DsbA
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E. coli GrpE
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E. coli Antibody
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E. coli Antibody
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E. coli Antibody
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E. coli Antibody
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E. coli Antibody
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E. coli Antibody
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E. coli (SLT-2, STX-2) Antibody
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E. coli STX 1 & 2 Antibody
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Inactivated DENV-2 Antigen (E. coli)
VAng-Lsx0084-1mg 1 mg
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Description: DENV-2, natural antigen from E. coli.

BMP-2 (E. coli-expressed), CF
PR15014CF 10 ug
EUR 311

Escherichia coli (E. coli) Antibody (Biotin)
abx411321-1ml 1 ml
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Escherichia coli (E. coli) Antibody (FITC)
abx411322-1ml 1 ml
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Escherichia coli (E. coli) Antibody (Biotin)
abx415713-1ml 1 ml
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Recombinant HIV-2 E gp 36 Protein (E. coli)
VAng-0534Lsx-inquire inquire Ask for price
Description: HIV type 2 Envelope glycoprotein 36, recombinant protein from E. coli. MW 16 kDa, 1 mg/mL.

Recombinant (E. coli) Dengue Virus Type 2 (and epitopes for type 1, 2, and 3) E Antigen (DENV E) (23 kda, >95% pure)
RP-1598 50 ug
EUR 408

Benzonase, E. Coli Recombinant
7680-25
EUR 332

Benzonase, E. Coli Recombinant
7680-5
EUR 137

E. Coli HCP antibody
20R-EG001 1 ml
EUR 837
Description: Goat polyclonal E. Coli HCP antibody

E. coli O157 antibody
10C-CR1295M3 100 ug
EUR 392
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody
10-1490 100 ug
EUR 136
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody
10-1521 100 ug
EUR 142
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody
10-E12A 100 ug
EUR 385
Description: Mouse monoclonal E. coli O157 antibody

TesA, E. coli esterase
0306-1 100 Units
EUR 421

TesA, E. coli esterase
0306-5 500 Units
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E. Coli Recombinant TRXB
6331-100
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E. Coli Recombinant SlyD
6366-100
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E. coli antibody (FITC)
60-E13 1 ml
EUR 378
Description: Rabbit polyclonal E. coli antibody (FITC) conjugated

E. coli antibody (biotin)
60-E13B 1 ml
EUR 468
Description: Rabbit polyclonal E. coli antibody (biotin) conjugated

E. coli antibody (HRP)
60-E14 1 ml
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Description: Rabbit polyclonal E. coli antibody (HRP) conjugated

E. coli Recombinant IDNK
P1050-10
EUR 164

E. coli Recombinant IDNK
P1050-50
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Recombinant E. coli, Glucokinase
P1054-10
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Recombinant E. coli, Glucokinase
P1054-50
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E. coli DNA Ligase
M1217-100
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E. coli Exonuclease I
M1222-100
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E. coli Exonuclease III
M1223-100
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RecA Protein E. coli
M1231-200
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E. coli FimC [His]
DAG1666 100 ug
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E. coli Eco [His]
DAG1667 50 ug
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E. coli O157 Antibody
abx021684-1mg 1 mg
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E. coli O157 Antibody
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E. coli Antibody (HRP)
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abx024017-1mg 1 mg
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E. coli O 157
abx291002-100mg 100 mg
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E. coli Topoisomerase IV
TG2000EC-1 250 units
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E. coli Topoisomerase IV
TG2000EC-3 500 units
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E. coli Topoisomerase IV
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E. coli DNA Gyrase
TG2000G-1 100 units
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E. coli DNA Gyrase
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E. coli DNA Gyrase
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E. coli DNA Gyrase
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E. coli CysH Antibody
30281-05111 150 ug
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E. coli DnaJ Antibody
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E. coli pykF Antibody
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E. coli GOR Antibody
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E. coli NDK Antibody
32662-05111 150 ug
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E. coli PPA Antibody
32678-05111 150 ug
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E. coli glk Antibody
32864-05111 150 ug
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E. Coli ldhA Antibody
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E. coli Skp Antibody
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E. coli nanA Antibody
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E. coli RecA Protein
01-001 200 ug
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Description: The E. coli RecA Protein is available in Europe and for worldwide shipping via Gentaur.

E. coli LexA repressor
01-005 50 ug
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Description: The E. coli LexA repressor is available in Europe and for worldwide shipping via Gentaur.

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01-006 250 ug
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E. coli RuvA protein
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E. coli RuvA protein
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E. coli RuvB protein
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E. coli RuvC protein
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E. coli RuvC protein
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Enterotoxin STp (E. coli)
H-6248.0500 0.5mg
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Description: Sum Formula: C81H110N20O26S6; CAS# [115474-04-9] net

Enterotoxin STp (E. coli)
H-6248.1000 1.0mg
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Recombinant (E. coli) Human Tumour Type M2 Pyruvate Kinase
RP-744 10 ug
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E. coli TrxA (aa 2 - 109) [His]
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E. coli Shiga toxin 2 PCR kit
PCR-VH108-48D 50T
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E. coli O157 Verotoxin 2 (VT2) Protein
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Recombinant HSV-2 gD Protein (E. coli)
VAng-0548Lsx-inquire inquire Ask for price
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E. coli Carbonic Anhydrase 2 (CA2) Antibody
30109-05111 150 ug
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Human Escherichia coli (E. coli) ELISA Kit
abx052493-96tests 96 tests
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Cow Escherichia coli (E. coli) ELISA Kit
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MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody
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MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody
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MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody
abx034997-400ul 400 ul
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MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody
abx034997-80l 80 µl
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MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody
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MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody
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MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody
20-abx001036
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MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody
abx026331-400ul 400 ul
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MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody
abx026331-80l 80 µl
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MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody
abx235371-100ug 100 ug
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MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody
abx235372-100ug 100 ug
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MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody
abx331633-100ul 100 ul
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MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli) (MSH2) Antibody
20-abx326761
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ELISA kit for Human RAG-2 (Recombination Activating Gene 2)
E-EL-H2320 1 plate of 96 wells
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Description: A sandwich ELISA kit for quantitative measurement of Human RAG-2 (Recombination Activating Gene 2) in samples from Serum, Plasma, Cell supernatant

E. Coli Proteins-Agarose affinity gel for removing E. coli antibodies
EC11-G 1 ml
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Coelenterazine e
10124-2 250uG
EUR 399
Description: Minimum order quantity: 1 unit of 250uG

Human pre-microRNA Expression Construct 146a-2
PMIRH146a-PA-2 Bacterial Streak
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Escherichia coli Serotype O2 (E. coli O2) Antibody
abx411330-02mg 0.2 mg
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Escherichia coli Serotype O15 (E. coli O15) Antibody
abx411331-02mg 0.2 mg
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Escherichia coli Serotype O20 (E. coli O20) Antibody
abx411332-02mg 0.2 mg
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E. coli O157:H7 antibody
70-XG13 1 mg
EUR 612
Description: Affinity purified Goat polyclonal E. coli O157:H7 antibody. 

Insulin, human recombinant (E. coli)
4772-25
EUR 452

Insulin, human recombinant (E. coli)
4772-5
EUR 207

E. coli antibody (K99 Pilus)
10-E20A 500 ug
EUR 493
Description: Mouse monoclonal E. coli antibody (K99 Pilus)

E. coli O157 antibody (FITC)
61C-CR1295M1F 100 ug
EUR 408
Description: Mouse monoclonal E. coli O157 antibody (FITC)

E. coli O157 antibody (FITC)
61C-CR1295M4F 100 ug
EUR 468
Description: Mouse monoclonal E. coli O157 antibody (FITC)

E. Coli Recombinant Thioredoxin 1
6329-50
EUR 403

E. coli Recombinant Carbonic anhydrase
P1049-10
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E. coli Recombinant Carbonic anhydrase
P1049-50
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E. coli DNA Polymerase I
M1210-100
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E. coli β-gal peptide
DAG2441 0.05 mg
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Native E. Coli O 158
DAGA-3025 0.1 ml
EUR 715

Uricase from E. coli, Recombinant
DIA-415 5KU
EUR 378

Recombinant Diacylglycerol Kinase (E. coli)
MET-5035 100 mg
EUR 276
Description: Purified recombinant prokaryotic DAG kinase expressed in E. coli

Recombinant Diacylglycerol Kinase (E. coli)
MET-5035-5 5 x 100 mg
EUR 920
Description: Purified recombinant prokaryotic DAG kinase expressed in E. coli

E. Coli O 157 Protein
abx061475-01ml 0.1 ml
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E. coli Enterotoxin B Antibody
abx021678-1mg 1 mg
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E. coli Enterotoxin B Antibody
abx021679-1mg 1 mg
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E. coli Enterotoxin B Antibody
abx021680-1mg 1 mg
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E. coli O157 Antibody (FITC)
abx021683-1mg 1 mg
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E. coli O157:H7 Antibody
abx022980-1mg 1 mg
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E. coli Enterotoxin LT Antibody
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E. coli Enterotoxin LT Antibody
abx023835-1mg 1 mg
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E. coli Enterotoxin LT Antibody
abx023836-1mg 1 mg
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Utilizing three completely different plasmid DNA codings for kanamycin (KM), chloramphenicol (CP), and ampicillin- (AMP) and tetracycline- (TC) resistance, 4 completely different competent Escherichia coli strains had been reworked by the calcium chloride methodology to provide KM-, CP- and AMP- and TC-resistant strains. Analysis of minimal inhibitory concentrations (MIC) of 22 antibiotics, confirmed KM-resistant E. coli to be cross resistant solely to fradiomycin (FRM); CP-resistant E. coli, particularly HB101 and JM109 strains, exhibited cross-resistance solely to thiamphenicol (TP). However, AMP- and TC-resistant E. coli confirmed cross resistance to a number of penicillins, tetracyclines and erythromycin.

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