BioConstructor Molecular Cloning Software

Lenti vector viral particles from E. coli plasmids with Puro

Diverse Fluoroquinolone Resistance Plasmids From Retail Meat E. coli in the United States.

Diverse Fluoroquinolone Resistance Plasmids From Retail Meat E. coli in the United States.

Fluoroquinolones are used to deal with severe bacterial infections, together with these brought on by Escherichia coli and Salmonella enterica. The emergence of plasmid-mediated quinolone resistance (PMQR) symbolize a brand new problem to the profitable therapy of Gram-negative infections. As a part of a long-term technique to generate a reference database of closed plasmids from antimicrobial resistant foodborne micro organism, we carried out long-read sequencing of 11 E. coli isolates from retail meats that had been non-susceptible to ciprofloxacin. Every of the isolates had PMQR genes, together with qnrA1qnrS1, and qnrB19.

The 4 qnrB19 genes had been carried on two distinct ColE-type plasmids amongst isolates from pork chop and floor turkey and had been similar to plasmids beforehand recognized in Salmonella. Seven different plasmids differed from every other sequences in GenBank and comprised IncF and IncR plasmids that ranged in measurement from 48 to 180 kb. These plasmids additionally contained completely different mixtures of resistance genes, together with these conferring resistance to beta-lactams, macrolides, sulfonamides, tetracycline, and heavy metals.

Though comparatively few isolates have PMQR genes, the identification of various plasmids in a number of retail meat sources suggests the potential for additional unfold of fluoroquinolone resistance, together with by way of co-selection. These outcomes spotlight the worth of long-read sequencing in characterizing antimicrobial resistance genes of public well being concern. We additional present that these unusually excessive lactose uptake charges are poisonous to the cells, resulting in elevated cell leakiness and lysis. Lastly, we exhibit that in distinction to plasmid-based T7 expression methods, IPTG induction is useful for genome-integrated T7 expression methods regarding cell health and productiveness.

Identification of σE-Dependent Promoter Upstream of clpB from the Pathogenic Spirochaete Leptospira interrogans by Making use of an Ecoli Two-Plasmid System.

There may be restricted info on gene expression within the pathogenic spirochaete Leptospirainterrogans and genetic mechanisms controlling its virulence. Transcription is step one in gene expression that’s typically decided by environmental results, together with infection-induced stresses. Alterations within the surroundings lead to important modifications within the transcription of many genes, permitting efficient adaptation of Leptospira to mammalian hosts. Thus, promoter and transcriptional begin web site identification are essential for figuring out gene expression regulation and for the understanding of genetic regulatory mechanisms current in Leptospira.

Right here, we characterised the promoter area of the L. interrogans clpB gene (clpBLi) encoding an AAA+ molecular chaperone ClpB important for the survival of this spirochaete beneath thermal and oxidative stresses, and likewise throughout an infection of the host. Primer extension evaluation demonstrated that transcription of clpB in L. interrogans initiates at a cytidine situated 41 bp upstream of the ATG initiation codon, and, to a lesser extent, at an adenine situated 2 bp downstream of the recognized web site. Transcription of each transcripts was heat-inducible.

Dedication of clpBLi transcription begin web site, mixed with promoter transcriptional exercise assays utilizing a modified two-plasmid system in E. coli, revealed that clpBLi transcription is managed by the ECF σE issue. Of the ten Linterrogans ECF σ components, the issue encoded by LIC_12757 (LA0876) is almost certainly to be the important thing regulator of clpB gene expression in Leptospira cells, particularly beneath thermal stress. Moreover, clpB expression could also be mediated by ppGpp in Leptospira.

Diverse Fluoroquinolone Resistance Plasmids From Retail Meat E. coli in the United States.

Figuring out the Virulence Properties of Escherichia coli ST131 Containing Bacteriocin-Encoding Plasmids Utilizing Quick- and Lengthy-Learn Sequencing and Evaluating Them with These of Different Ecoli Lineages.

Escherichia coli ST131 is a scientific problem as a consequence of its multidrug resistant profile and profitable international unfold. They’re typically related to difficult infections, significantly urinary tract infections (UTIs). Bacteriocins play an vital function to outcompete different microorganisms current within the human intestine. Right here, we characterised bacteriocin-encoding plasmids present in ST131 isolates of sufferers affected by a UTI utilizing each short- and long-read sequencing. Colicins Ia, Ib and E1, and microcin V, had been recognized amongst plasmids that additionally contained resistance and virulence genes.

To research if the potential transmission vary of the colicin E1 plasmid is influenced by the presence of a resistance gene, we constructed a pressure containing a plasmid which had each the colicin E1 and blaCMY-2 genes. No distinction in transmission vary was discovered between transformant and wild-type strains. Nonetheless, a statistically considerably distinction was present in adhesion and invasion capacity. Bacteriocin-producing isolates from each ST131 and non-ST131 lineages had been capable of inhibit the expansion of different E. coli isolates, together with different ST131. In abstract, plasmids harboring bacteriocins give extra benefits for extremely virulent and resistant ST131 isolates, enhancing the power of those isolates to compete with different microbiota for a distinct segment and thereby rising the danger of an infection.

The current examine aimed to judge the affect of induction situations (IPTG focus, temperature, and induction time) on the plasmid pQE-30 stability and 503 antigen expression of Leishmania i. chagasi in Escherichia coli M15. Batch cultures had been carried out at 37 °C and induced by the addition of various IPTG concentrations (0.01 to 1.5 mM). Subsequently, experiments had been carried out at completely different temperatures (27 to 42 °C), evaluating the affect of induction time (0.5 to six h after the beginning of the tradition). The outcomes confirmed that IPTG toxicity brought on a metabolic stress within the cells and, consequently, the microorganism development decreased.

PRDM13 Polyclonal Antibody

A-2013
  • EUR 754.26
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  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

PRDM16 Polyclonal Antibody

A-2016
  • EUR 754.26
  • EUR 117.70
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

PRDM17 Polyclonal Antibody

A-2017
  • EUR 754.26
  • EUR 117.70
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

EZH1 Polyclonal Antibody

A-2018
  • EUR 754.26
  • EUR 117.70
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

HSF1 Polyclonal Antibody

A-2401
  • EUR 905.64
  • EUR 235.40
  • EUR 423.50
  • EUR 574.20
  • 200 µl
  • 50 ul
  • 100 ul
  • 200 ul

PRMT3 Polyclonal Antibody

A-3003
  • EUR 740.34
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  • EUR 462.00
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

SET1 Polyclonal Antibody

A-3011
  • EUR 754.26
  • EUR 117.70
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

SET07 Polyclonal Antibody

A-3013
  • EUR 754.26
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  • Ask for price
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

LSD1 Polyclonal Antibody

A-3018
  • EUR 754.26
  • EUR 117.70
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

HDAC10 Polyclonal Antibody

A-4010
  • EUR 754.26
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  • EUR 470.80
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

PCAF Polyclonal Antibody

A-4012
  • EUR 740.34
  • EUR 117.70
  • EUR 306.90
  • EUR 462.00
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

Histone H3K9ac (Acetyl H3K9) Polyclonal Antibody

A-4022
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K14ac (Acetyl H3K14) Polyclonal Antibody

A-4023
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K18ac (Acetyl H3K18) Polyclonal Antibody

A-4024
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K23ac (Acetyl H3K23) Polyclonal Antibody

A-4025
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 383.90
  • 50 µg
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K56ac (Acetyl H3K56) Polyclonal Antibody

A-4026
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K4me1 (H3K4 Monomethyl) Polyclonal Antibody

A-4031
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K4me2 (H3K4 Dimethyl) Polyclonal Antibody

A-4032
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 383.90
  • 50 µg
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K4me3 (H3K4 Trimethyl) Polyclonal Antibody

A-4033
  • EUR 555.90
  • EUR 177.10
  • EUR 342.10
  • EUR 479.60
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K9me1 (H3K9 Monomethyl) Polyclonal Antibody

A-4034
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K9me2 (H3K9 Dimethyl) Polyclonal Antibody

A-4035
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K9me3 (H3K9 Trimethyl) Polyclonal Antibody

A-4036
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K27me1 (H3K27 Monomethyl) Polyclonal Antibody

A-4037
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K27me2 (H3K27 Dimethyl) Polyclonal Antibody

A-4038
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody

A-4039
  • EUR 555.90
  • EUR 177.10
  • EUR 342.10
  • EUR 479.60
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K36me1 (H3K36 Monomethyl) Polyclonal Antibody

A-4040
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K36me2 (H3K36 Dimethyl) Polyclonal Antibody

A-4041
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K36me3 (H3K36 Trimethyl) Polyclonal Antibody

A-4042
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K79me1 (H3K79 Monomethyl) Polyclonal Antibody

A-4043
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K79me2 (H3K79 Dimethyl) Polyclonal Antibody

A-4044
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K79me3 (H3K79 Trimethyl) Polyclonal Antibody

A-4045
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H4K20me1 (H4K20 Monomethyl) Polyclonal Antibody

A-4046
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H4K20me2 (H4K20 Dimethyl) Polyclonal Antibody

A-4047
  • EUR 555.90
  • EUR 173.80
  • EUR 336.60
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H4K20me3 (H4K20 Trimethyl) Polyclonal Antibody

A-4048
  • EUR 555.90
  • EUR 173.80
  • EUR 302.50
  • EUR 470.80
  • 50 µl
  • 25 ul
  • 50 ul
  • 100 ul

Histone H3K27ac (Acetyl H3K27) Polyclonal Antibody

A-4708
  • EUR 813.42
  • EUR 173.80
  • EUR 299.20
  • EUR 510.40
  • 100 µl
  • 25 ul
  • 50 ul
  • 100 ul

CRISPR Cas9 Monoclonal Antibody [7A9]

A-9000
  • EUR 573.30
  • EUR 71.50
  • EUR 200.20
  • EUR 355.30
  • 100 µg
  • 10 ug
  • 50 ug
  • 100 ug

CRISPR/Cas9 (SaCas9) Monoclonal Antibody [6H4]

A-9001
  • EUR 559.38
  • EUR 70.40
  • EUR 192.50
  • EUR 338.80
  • 100 µg
  • 10 ug
  • 50 ug
  • 100 ug

CLIMP-63 Polyclonal Antibody

A-0701
  • EUR 754.26
  • EUR 290.40
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

DNMT3A Polyclonal Antibody

A-1003
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

DNMT3B Polyclonal Antibody

A-1004
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

DNMT3L Polyclonal Antibody

A-1005
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

MBD3 Polyclonal Antibody

A-1008
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

MGMT Polyclonal Antibody

A-1010
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

MeCP2 Polyclonal Antibody

A-1012
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

TET1 Polyclonal Antibody

A-1020
  • EUR 628.98
  • EUR 221.10
  • EUR 386.10
  • 100 µl
  • 50 ul
  • 100 ul

DNMT1 Polyclonal Antibody

A-1700
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

TET2 Polyclonal Antibody

A-1701
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

MBD2 Polyclonal Antibody

A-1713
  • EUR 736.86
  • EUR 281.60
  • EUR 459.80
  • 100 µl
  • 50 ul
  • 100 ul

PRDM2 Polyclonal Antibody

A-2002
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  • EUR 117.70
  • EUR 284.90
  • 100 µg
  • 20 ul
  • 50 ul

PRDM3 Polyclonal Antibody

A-2003
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

PRDM6 Polyclonal Antibody

A-2006
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

PRDM12 Polyclonal Antibody

A-2012
  • EUR 754.26
  • EUR 284.90
  • EUR 383.90
  • 100 µg
  • 50 ul
  • 100 ul

PRDM14 Polyclonal Antibody

A-2014
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

EZH2 Polyclonal Antibody

A-2019
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

EED Polyclonal Antibody

A-2020
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

Swi2/SNF2 Polyclonal Antibody

A-2023
  • EUR 742.08
  • EUR 303.60
  • EUR 463.10
  • 100 µl
  • 50 ul
  • 100 ul

SNFa/BRM Polyclonal Antibody

A-2025
  • EUR 740.34
  • EUR 235.40
  • EUR 383.90
  • 100 µg
  • 50 ul
  • 100 ul

Ini1 Polyclonal Antibody

A-2026
  • EUR 740.34
  • EUR 235.40
  • EUR 383.90
  • 100 µg
  • 50 ul
  • 100 ul

RNA Polymerase II Monoclonal Antibody [CTD4H8]

A-2032
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µg
  • 50 ug
  • 100 ug

CBX5 Polyclonal Antibody

A-2701
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

EGLN1 Polyclonal Antibody

A-2702
  • EUR 580.26
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  • 100 µl
  • 50 ul
  • 100 ul

RBBP4 Polyclonal Antibody

A-2703
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SIN3A Polyclonal Antibody

A-2704
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

CTBP1 Polyclonal Antibody

A-2705
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

PADI4 Polyclonal Antibody

A-2706
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SMARCA5 Polyclonal Antibody

A-2707
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

IDH1 Polyclonal Antibody

A-2708
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

CHD4 Polyclonal Antibody

A-2709
  • EUR 535.02
  • EUR 322.30
  • EUR 423.50
  • 50 µl
  • 50 ul
  • 100 ul

CHAF1A Polyclonal Antibody

A-2710
  • EUR 535.02
  • EUR 322.30
  • EUR 423.50
  • 50 µl
  • 50 ul
  • 100 ul

BRD7 Polyclonal Antibody

A-2712
  • EUR 535.02
  • EUR 328.90
  • EUR 432.30
  • 50 µl
  • 50 ul
  • 100 ul

UHRF1 Polyclonal Antibody

A-2714
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

UHRF2 Polyclonal Antibody

A-2715
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SMARCE1 Polyclonal Antibody

A-2716
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

ACTL6A Polyclonal Antibody

A-2717
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

MPG Polyclonal Antibody

A-2718
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

HIF1AN Polyclonal Antibody

A-2719
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

RNF2 Polyclonal Antibody

A-2720
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

PCGF6 Polyclonal Antibody

A-2721
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SMARCB1 Polyclonal Antibody

A-2722
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

ALKBH3 Polyclonal Antibody

A-2723
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

BBOX1 Polyclonal Antibody

A-2724
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

BTAF1 Polyclonal Antibody

A-2725
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

CXXC1 Polyclonal Antibody

A-2726
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

KDM4B Polyclonal Antibody

A-2727
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

HELLS Polyclonal Antibody

A-2728
  • EUR 742.08
  • EUR 303.60
  • EUR 463.10
  • 100 µl
  • 50 ul
  • 100 ul

ING3 Polyclonal Antibody

A-2729
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

ING4 Polyclonal Antibody

A-2730
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

PHC1 Polyclonal Antibody

A-2731
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SMARCAD1 Polyclonal Antibody

A-2732
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

USP16 Polyclonal Antibody

A-2733
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SMARCC1 Polyclonal Antibody

A-2734
  • EUR 742.08
  • EUR 303.60
  • EUR 463.10
  • 100 µl
  • 50 ul
  • 100 ul

ERCC6L Polyclonal Antibody

A-2735
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SMYD5 Polyclonal Antibody

A-2736
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

ATF2 Polyclonal Antibody

A-2738
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

BMI1 Polyclonal Antibody

A-2739
  • EUR 869.10
  • Ask for price
  • Ask for price
  • 100 µl
  • 50 ul
  • 100 ul

CTBP2 Polyclonal Antibody

A-2740
  • EUR 869.10
  • EUR 322.30
  • EUR 548.90
  • 100 µl
  • 50 ul
  • 100 ul

CTCF Polyclonal Antibody

A-2741
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

MTA2 Polyclonal Antibody

A-2742
  • EUR 869.10
  • EUR 322.30
  • EUR 548.90
  • 100 µl
  • 50 ul
  • 100 ul

PMS1 Polyclonal Antibody

A-2744
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SFN Polyclonal Antibody

A-2745
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SMARCA4 Polyclonal Antibody

A-2746
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

SMARCAL1 Polyclonal Antibody

A-2747
  • EUR 869.10
  • Ask for price
  • Ask for price
  • 100 µl
  • 50 ul
  • 100 ul

SMARCC2 Polyclonal Antibody

A-2748
  • EUR 580.26
  • EUR 223.30
  • EUR 353.10
  • 100 µl
  • 50 ul
  • 100 ul

MuERVL-Gag Polyclonal Antibody

A-2801
  • EUR 684.66
  • EUR 239.80
  • EUR 432.30
  • 100 µl
  • 50 ul
  • 100 ul

PRMT1 Polyclonal Antibody

A-3001
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

PRMT2 Polyclonal Antibody

A-3002
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

PRMT4 Polyclonal Antibody

A-3004
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

PRMT5 Polyclonal Antibody

A-3005
  • EUR 740.34
  • EUR 235.40
  • EUR 462.00
  • 100 µl
  • 50 ul
  • 100 ul

PRMT7 Polyclonal Antibody

A-3007
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

SUV39H2 Polyclonal Antibody

A-3010
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

SET9 Polyclonal Antibody

A-3014
  • EUR 754.26
  • EUR 284.90
  • EUR 470.80
  • 100 µl
  • 50 ul
  • 100 ul

The induction with IPTG may additionally be related to the plasmid pQE-30 instability, as a consequence of metabolic burden imposed by the recombinant protein expression. The optimum situations for 503 antigen expression of Leishmania i. chagasi in Escherichia coli M15 had been an IPTG focus of 1.Zero mM, temperature of 37 °C, and induction time of two h. The utmost antigen focus obtained was 0.119 ± 0.009 g/L, about seven occasions greater than the bottom focus. Due to this fact, the outcomes confirmed that 503 antigen will be produced in laboratory; nonetheless, it requires extra research to attenuate the plasmid instability and enhance to industrial scale.

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