BioConstructor Molecular Cloning Software

Lenti vector viral particles from E. coli plasmids with Puro

A two-plasmid system for identification of promoters recognized by RNA polymerase containing extracytoplasmic stress response sigma(E) in Escherichia coli.

A two-plasmid system for identification of promoters recognized by RNA polymerase containing extracytoplasmic stress response sigma(E) in Escherichia coli.

We have now beforehand established a two-plasmid system in Escherichia coli for identification of promoters acknowledged by RNA polymerase containing a heterologous sigma issue. Makes an attempt to optimize this method for identification of promoters acknowledged by RNA polymerase containing E. coli extracytoplasmic stress response sigma(E) failed owing to excessive toxicity of the expressed rpoE. A brand new system for identification of sigma(E)-cognate promoters was established, and verified utilizing the 2 recognized sigma(E)-dependent promoters, rpoEp2 and degPp. Expression of the sigma(E)-encoding rpoE gene was underneath the management of the AraC-dependent P(BAD) promoter.

A low stage of arabinose induced a non-toxic, nevertheless, ample stage of sigma(E) to work together with the core enzyme of RNA polymerase. Such an RNA polymerase holoenzyme acknowledged each recognized sigma(E)-dependent promoters, rpoEp2 and degPp, which have been cloned within the appropriate promoter probe plasmid, upstream of a promoterless lacZ alpha reporter gene. This new system has proved to be helpful for identification of E. coli sigma(E)-cognate promoters. Furthermore, the system could possibly be used for identification of ECF sigma-cognate promoters from different micro organism.

An built-in flow-injection processing (FIP) system for the quantification of plasmids throughout cultivation is described. The system performs on-line sampling, cell lysis, and quantification of plasmids in an built-in method throughout cultivation of E. coli. The system was operated through the use of a miniaturized expanded-bed column which can be utilized for dealing with samples containing cells and cell particles with out interfering with the binding evaluation. Two varieties of detectors (one measuring UV absorbance at 254 nm and a fluorometer) are used for on-line plasmid detection. The system was developed utilizing customary options and it was efficiently utilized in monitoring plasmid contents throughout a cultivation of E. coli.

Complementation between dimeric mutants as a probe of dimer-dimer interactions in tetrameric dihydrofolate reductase encoded by R67 plasmid of Ecoli.

The impact of mutations on the interactions between dimers in R67 dihydrofolate reductase (R67 DHFR), a tetrameric enzyme conferring resistance to trimethoprim, was investigated by site-directed mutagenesis mixed with phenotypic, enzymatic, and biochemical evaluation. Some 14 mutants at two positions concerned in a hydrogen bond between dimers have been constructed. All have been proven to be dimers. Nevertheless, complementation between pairs of dimeric mutated proteins resulted within the restoration of the enzymatic exercise and heterotetramer formation. A combinatorial method was set as much as create effectively such heterotetramers and establish the complementing pairs of mutations. A dozen of such pairs have been discovered.

An correct methodology was set as much as measure the affiliation of the complementing dimers in a “quasi-isologous” heterotetramer and used to review the consequences of mutations and pH on the affiliation. Thus, the pair of proteins bearing respectively the S59A and H62L mutations was proven to type heterotetramers with catalytic properties near these of the wild-type protein. Its affiliation was as robust as that of the wild-type protein at cytoplasmic pH (6. 5), and was extra steady at decrease pH values.A double-mutant protein bearing concurrently the S59A and H62L mutations was produced and analyzed.

Its affiliation was weakened by 1.2 kcal/mol as in comparison with the wild-type enzyme at pH 6.5 however was insensitive to pH. Evaluating the vitality of affiliation between dimers within the wild-type protein, the heterotetramer and the double mutant allowed us to dissect the consequences of the pH and of the molecular context on a subset of interactions between the R67 DHFR subunits.

A two-plasmid system for identification of promoters recognized by RNA polymerase containing extracytoplasmic stress response sigma(E) in Escherichia coli.

Immunoadjuvant actions of Ecoli– and plasmid-expressed recombinant hen IFN-alpha/beta, IFN-gamma and IL-1beta in 1-day- and 3-week-old chickens.

Within the current examine we assessed the capability of recombinant E. coli- or plasmid-expressed hen interferons (IFN) and hen IL-1beta, to exert immunostimulatory actions for humoral immune responses, in day-old and grownup chickens. We noticed that each recombinant E. coli-expressed hen IFN-alpha/beta and IFN-gamma facilitated the induction of a major and in addition a secondary antibody response, utilizing tetanus toxoid (TT) as a bacterial mannequin antigen, in immunologically mature 3-week-old chickens. In distinction, no enchancment of antibody both kind of hen IFN was co-injected with inactivated Infectious Bursal Illness Virus (IBDV) antigen.

TT-specific antibody formation was marginally elevated by co-injection of recombinant E. coli-expressed hen IL-1beta. Mixed administration of IFN-alpha/beta plus IFN-gamma or IL-beta elevated responses to TT in an additive, however not synergistic style. Remarkably, no augmentation of antibody responses particular for TT, nor IBDV, was famous in day-old birds, receiving IFN-alpha/beta or IFN-gamma as adjuvant. Additionally, intramuscular immunization of 3-week-old birds, utilizing plasmids encoding IFN-alpha/beta along with TT protein antigen, considerably elevated the pace and magnitude of TT-specific antibody responses. Plasmids encoding hen IL-beta or IFN-gamma had a minimal or inhibitory impact, respectively. These knowledge point out a possible for hen cytokines as immunoadjuvant for explicit varieties of hen vaccine antigens.

E. coli RecQ DNA helicase

01-004 250? g
EUR 1858.8
Description: The E. coli RecQ DNA helicase is available in Europe and for worldwide shipping via Gentaur.

E. coli DNA Polymerase I

M1210-100
EUR 307.2

pBR322

G30-305 200µg
EUR 169.2

pBR322

G30-325 5x200µg
EUR 660

E. coli DNA polymerase Assay Kit Plus (E. coli DNA pol III alpha subunit included)

DPA100KE 100 assays
EUR 444.91
Description: This product includes all the reagents in the DNA Polymerase III Alpha Assay Kit (Catalog No. DPA100K) plus 35 ul of 100 x E. coli DNA polymerase III alpha subunit.

E. coli DNA Primase Assay Kit Plus (E. coli primase-helicase included)

EGA100KE 100 assays
EUR 444.91
Description: This product includes all the kit components in E. coli DNA Primase Assay Kit (Catalog No. EGA100K ) plus 45 ul of 100 x E. coli primase-helicase complex. It is for 100 assays of E.coli DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format.

Uracil DNA Glycosylase Modified, from E. coli

GZ6540-500IU 500 IU
EUR 322.8

Uracil DNA Glycosylase Modified, from E. coli

GZ6540-5KIU 5 KIU
EUR 1328.4

T4 DNA Ligase, expressed in E. coli

GZ9900-1KIU 1 KIU
EUR 217.2

T4 DNA Ligase, expressed in E. coli

GZ9900-5KIU 5 KIU
EUR 562.8

E. coli DNA helicase - for 100 assays

DNAB-100EC 100 assays
EUR 309.4
Description: This product includes 33 ul of 20 uM E. coli DNA helicase.

E. coli DNA primase - for 100 assays

DNAG-100EC 100 assays
EUR 309.4
Description: This product includes 45 ul of 10 uM E. coli primase-helicase complex

E. coli DNA ligase - for 100 assays

LIG-100EC 100 assays
EUR 309.4
Description: This product includes 10 ul of 500 nM E. coli ligase.

E. coli DNA gyrase - for 100 assays

TOP2-100EC 100 assays
EUR 309.4
Description: This product includes wild-type E. coli gyrase for 100 assays (40 ul of 2000 nM stock solution)

E. coli DNA Topoisomerase IV Assay Kit Plus (E. coli Topo IV included)

DDC020KE 20 assays
EUR 221.78
Description: This product includes all the components in DNA Topoisomerase IV Assay Kit (Catalog No. DDC020K)plus 12ul of 100 x E.coli topoisomerase IV (500 nM) for 20 E. coli topoisomerase IV DNA decatenation assays.

E. coli DNA Topoisomerase I Assay Kit Plus (E. coli Topo I included)

DRA020KE 20 assays
EUR 221.78
Description: This product includes 160 ul of 10 x Buffer T1, 84 ul of 10 x supercoiled plasmid DNA, 10 ul of 100 x E. coli topoisomerase I, 8 ul of 1500 x Dye H19 and 500 ul of 10 x H19 dilution buffer. It is for 20 DNA relaxation assays in a 96-well plate format or 40 assays in a 384-well assay format.

E. coli DNA Topoisomerase II (Gyrase) Assay Kit Plus (E. coli gyrase included)

DSA020KE 20 assays
EUR 221.78
Description: This product includes160 ul of 10 x Buffer T2, 84 ul of 10 x relaxed DNA, 6 ul of 1500 x H19 dye, 90 ul of 10 x ATP and 550 ul of 10 x H19 dilution buffer. It is for 20 assays in 96-well plate format. E. coli gyrase is not included.

E. coli NAD+-dependent DNA ligase assay kit Plus (E. coli ligase included)

NLA100KE 100 assays
EUR 444.91
Description: This product includes 800 ul of 10 x buffer, 44 ul of 100x DNA substrate, 44 ul of 100 x NAD+, 21 ml of Reagent T, 10 ul of 500 x E. coli ligase and 220 ul of 10 x fluorescence dye for 100 assays.

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N150 500 units
EUR 122.4

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N152 5x500 units
EUR 472.8

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)

N154 20x500 units
EUR 1830

E. coli DNA polymerase alpha - for 100 assays

DPA-100EC 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM E. coli DNA polymerase III alpha.

E. coli DNA topoisomerase I - for 100 assays

TOP1-100EC 100 assays
EUR 309.4
Description: This product includes 42 ul of 4 uM E. coli topoisomerase I

Escherichia coli (E. coli) Antibody

abx415692-01mg 0.1 mg
EUR 760.8

Escherichia coli (E. coli) Antibody

abx415712-1ml 1 ml
EUR 727.2

Escherichia coli (E. coli) Antibody

abx411320-1ml 1 ml
EUR 610.8

E. coli antibody

10-E09C 100 ug
EUR 159.6
Description: Mouse monoclonal E. coli antibody

E. coli antibody

10-E10A 500 ug
EUR 591.6
Description: Mouse monoclonal E. coli antibody

E. coli antibody

10-E10B 500 ug
EUR 591.6
Description: Mouse monoclonal E. coli antibody

E. coli antibody

20C-CR1244GP 1 ml
EUR 561.6
Description: Goat polyclonal E. coli antibody

E. coli antibody

20-ER13 1 ml
EUR 426
Description: Rabbit polyclonal E. coli antibody

E. coli antibody

10C-CR1244M1 100 ug
EUR 489.6
Description: Mouse monoclonal E. coli antibody

E. coli Antibody

abx021669-1mg 1 mg
EUR 1554

E. coli Antibody

abx021670-1mg 1 mg
EUR 1045.2

E. coli Antibody

abx021671-02ml 0.2 ml
EUR 594

E. coli Antibody

abx021672-1mg 1 mg
EUR 1045.2

E. coli Antibody

abx021673-1mg 1 mg
EUR 1045.2

E. coli Antibody

abx022970-1ml 1 ml
EUR 878.4

E. coli Antibody

abx022971-1ml 1 ml
EUR 1161.6

E. coli Antibody

abx022975-1ml 1 ml
EUR 718.8

E. coli Antibody

abx022977-1ml 1 ml
EUR 811.2

E. coli GroL

DAG1704 100 ug
EUR 1098

E. coli GroS

DAG1705 100 ug
EUR 1098

E. coli DsbA

DAG1712 100 ug
EUR 1098

E. coli GrpE

DAG1714 100 ug
EUR 1232.4

Crude Extracellular Mixture of E. coli, DNA Aptamer, Biotinylated

AD-112-B Custom Ask for price

Crude Extracellular Mixture of E. coli, DNA Aptamer, unlabeled

AD-112-U Custom Ask for price

E. coli DNA Helicase ATPase assay Kit Plus-100

DNAB100KE 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x E. coli DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

E. coli DNA Helicase ATPase assay Kit Plus-500

DNAB500KE 500 assays
EUR 1396.74
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x E. coli DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

E. coli DNA Primase Assay Kit (enzyme not included)

EGA100K 100 assays
EUR 278.4
Description: This product includes 600 ul of 10 x assay buffer, 45 ul of 100 x DNA template, 45 ul of 100 x NTP mix and 850 ul of 10 x fluorescence dye for 100 assays of E. coli DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format. The kit does not include the enzyme.

E. coli single-strand DNA binding protein - 100 ug

SSB-100EC 100 ug
EUR 234.99
Description: This product includes 100 ug of E. coli single strand DNA binding protein

100 BP DNA LADDER, 500UL PER KIT

M-DNA-100BP 1/pk
EUR 87.6
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT

M-DNA-1KB 1/pk
EUR 84
Description: Bioscience Mol Bio; DNA Ladder

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (50ug Relaxed DNA + 100u Gyrase)

TG2000G-1KIT 100 assays
EUR 456

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (250ug Relaxed DNA + 500u Gyrase)

TG2000G-3KIT 500 assays
EUR 1182

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (500ug Relaxed DNA + 1000u Gyrase)

TG2000G-5KIT 1000 assays
EUR 1934.4

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (1mg Relaxed DNA + 2000u Gyrase)

TG2000G-7KIT 2000 assays
EUR 3412.8

Escherichia coli (E. coli) Antibody (Biotin)

abx415713-1ml 1 ml
EUR 861.6

Escherichia coli (E. coli) Antibody (Biotin)

abx411321-1ml 1 ml
EUR 710.4

Escherichia coli (E. coli) Antibody (FITC)

abx411322-1ml 1 ml
EUR 610.8

Purified E. coli DNA Gyrase Drug Screening Kit (50ug Relaxed DNA + 100u Gyrase)

TG2001G-1KIT 100 assays
EUR 496.8

Purified E. coli DNA Gyrase Drug Screening Kit (250ug Relaxed DNA + 500u Gyrase)

TG2001G-3KIT 500 assays
EUR 1262.4

Purified E. coli DNA Gyrase Drug Screening Kit (500ug Relaxed DNA + 1000u Gyrase)

TG2001G-5KIT 1000 assays
EUR 2203.2

Purified E. coli DNA Gyrase Drug Screening Kit (1mg Relaxed DNA + 2000u Gyrase)

TG2001G-7KIT 2000 assays
EUR 3547.2

Crude Extracellular Mixture of E. coli, DNA Aptamer, FITC labeled

AD-112-F Custom Ask for price

E. coli Topoisomerase IV Drug Screening Kit (25ug SC DNA)

TG1007-1 100 assays
EUR 388.8

E. coli Topoisomerase IV Drug Screening Kit (62.5ug SC DNA)

TG1007-2 250 assays
EUR 537.6

Gel-Based E. coli Topoisomerase I DNA relaxation Assay Kit

GDRA100KE 100 assays
EUR 399.28
Description: This product includes all the reagents for 100 samples in gel-based assays of E. coli topo I DNA relaxation activity. It This product includes 300 µl of 10 x Buffer T1, 100 µl of 250 mg/ml supercoiled DNA, 550 µl of 5 x Gel loading buffer and 10 µl of 4 µM E. coli topoisomerase I enzyme.

E. Coli Recombinant TRXB

6331-100
EUR 510

E. Coli Recombinant SlyD

6366-100
EUR 548.4

E. coli antibody (FITC)

60-E13 1 ml
EUR 453.6
Description: Rabbit polyclonal E. coli antibody (FITC) conjugated

E. coli antibody (biotin)

60-E13B 1 ml
EUR 561.6
Description: Rabbit polyclonal E. coli antibody (biotin) conjugated

E. coli antibody (HRP)

60-E14 1 ml
EUR 489.6
Description: Rabbit polyclonal E. coli antibody (HRP) conjugated

E. coli O157 antibody

10-1490 100 ug
EUR 163.2
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody

10-1521 100 ug
EUR 170.4
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody

10-E12A 100 ug
EUR 462
Description: Mouse monoclonal E. coli O157 antibody

E. coli DnaJ Antibody

32022-05111 150 ug
EUR 313.2

E. coli pykF Antibody

32163-05111 150 ug
EUR 313.2

E. coli GOR Antibody

32540-05111 150 ug
EUR 313.2

E. coli CysH Antibody

30281-05111 150 ug
EUR 313.2

E. Coli HCP antibody

20R-EG001 1 ml
EUR 1004.4
Description: Goat polyclonal E. Coli HCP antibody

E. coli NDK Antibody

32662-05111 150 ug
EUR 313.2

E. coli PPA Antibody

32678-05111 150 ug
EUR 313.2

E. coli glk Antibody

32864-05111 150 ug
EUR 313.2

E. Coli ldhA Antibody

32898-05111 150 ug
EUR 313.2

E. coli deoC Antibody

32936-05111 150 ug
EUR 313.2

E. coli Skp Antibody

32971-05111 150 ug
EUR 313.2

E. coli nanA Antibody

33092-05111 150 ug
EUR 313.2

E. coli O157 antibody

10C-CR1295M3 100 ug
EUR 470.4
Description: Mouse monoclonal E. coli O157 antibody

E. coli RecA Protein

01-001 200 ug
EUR 246
Description: The E. coli RecA Protein is available in Europe and for worldwide shipping via Gentaur.

E. coli LexA repressor

01-005 50 ug
EUR 574.8
Description: The E. coli LexA repressor is available in Europe and for worldwide shipping via Gentaur.

E. coli LexA repressor

01-006 250 ug
EUR 1420.8
Description: The E. coli LexA repressor is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvA protein

01-007 50 ug
EUR 574.8
Description: The E. coli RuvA protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvA protein

01-008 250 ug
EUR 1420.8
Description: The E. coli RuvA protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvB protein

01-009 50 ug
EUR 574.8
Description: The E. coli RuvB protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvB protein

01-010 250 ug
EUR 1420.8
Description: The E. coli RuvB protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvC protein

01-011 50 ug
EUR 574.8
Description: The E. coli RuvC protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvC protein

01-012 250 ug
EUR 1420.8
Description: The E. coli RuvC protein is available in Europe and for worldwide shipping via Gentaur.

TesA, E. coli esterase

0306-1 100 Units
EUR 505.2

TesA, E. coli esterase

0306-5 500 Units
EUR 2046

E. coli GroEL Antibody

20012-05011 150 ug
EUR 260.4

E. coli O157 Antibody

abx021684-1mg 1 mg
EUR 1220.4

E. coli O157 Antibody

abx021685-1mg 1 mg
EUR 1195.2

E. coli O157 Antibody

abx021686-1mg 1 mg
EUR 1195.2

E. coli O157 Antibody

abx021687-1mg 1 mg
EUR 1195.2

E. coli O157 Antibody

abx021688-1mg 1 mg
EUR 1128

E. coli O157 Antibody

abx021689-1mg 1 mg
EUR 1195.2

E. coli Antibody (HRP)

abx022972-1ml 1 ml
EUR 718.8

E. coli Antibody (FITC)

abx022974-1ml 1 ml
EUR 777.6

E. coli Antibody (Biotin)

abx022976-1ml 1 ml
EUR 718.8

E. coli Antibody (HRP)

abx022978-1ml 1 ml
EUR 1053.6

E. coli Antibody (Biotin)

abx022979-1ml 1 ml
EUR 886.8

E. coli O157 Antibody

abx024017-1mg 1 mg
EUR 1212

E. coli O 157

abx291002-100mg 100 mg
EUR 744

Benzonase, E. Coli Recombinant

7680-25
EUR 398.4

Benzonase, E. Coli Recombinant

7680-5
EUR 164.4

E. coli FimC [His]

DAG1666 100 ug
EUR 1232.4

E. coli Eco [His]

DAG1667 50 ug
EUR 1232.4

E. coli Exonuclease I

M1222-100
EUR 301.2

E. coli Exonuclease III

M1223-100
EUR 320.4

RecA Protein E. coli

M1231-200
EUR 242.4

E. coli Recombinant IDNK

P1050-10
EUR 196.8

E. coli Recombinant IDNK

P1050-50
EUR 457.2

Recombinant E. coli, Glucokinase

P1054-10
EUR 196.8

Recombinant E. coli, Glucokinase

P1054-50
EUR 457.2

Enterotoxin STp (E. coli)

H-6248.0500 0.5mg
EUR 835.2
Description: Sum Formula: C81H110N20O26S6; CAS# [115474-04-9] net

Enterotoxin STp (E. coli)

H-6248.1000 1.0mg
EUR 1416
Description: Sum Formula: C81H110N20O26S6; CAS# [115474-04-9] net

E. coli Topoisomerase IV

TG2000EC-1 250 units
EUR 537.6

E. coli Topoisomerase IV

TG2000EC-3 500 units
EUR 819.6

E. coli Topoisomerase IV

TG2000EC-5 1000 units
EUR 1262.4

E. coli O157 Antibody

V3307-100UG 100 ug
EUR 499
Description: This mAb shows specificity to E. coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.

E. coli O157 Antibody

V3307-20UG 20 ug
EUR 219
Description: This mAb shows specificity to E. coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.

E. coli O157 Antibody

V3307SAF-100UG 100 ug
EUR 499
Description: This mAb shows specificity to E. coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.

Human Escherichia coli (E. coli) ELISA Kit

abx052493-96tests 96 tests
EUR 801.6

Cow Escherichia coli (E. coli) ELISA Kit

abx055785-96tests 96 tests
EUR 801.6

E. Coli Proteins-Agarose affinity gel for removing E. coli antibodies

EC11-G 1 ml
EUR 343.2

E. coli DNA Topoisomerase IV Assay Kit Plus-100 (enzyme included)

DDC100KE 100 assays
EUR 681.29
Description: This product includes the 600 ul of assay buffer (Buffer T4), 520 ul of 10 x concatenated DNA, 120 ul of 50 x ATP (10 mM), 550 ul of 0.4 M EDTA, 780 ul of 20 x fluorescence dye, 100 spin columns and 55ul of 100 x E.coli topoisomerase IV (500 nM) for 100 E. coli topoisomerase IV DNA decatenation assays.

E. coli DNA Topoisomerase I Assay Kit Plus-100 (enzyme included)

DRA100KE 100 assays
EUR 590.51
Description: This product includes 480 ul of 10 x Buffer T1, 405 ul of 10 x supercoiled plasmid DNA, 20 ul of 1500 x Dye H19, 3000 ul of 10 x H19 dilution buffer and 42 ul of 100 x E. coli topoisomerase I. It is for 100 DNA relaxation assays in a 96-well plate format or 200 assays in a 384-well assay format

Gel-Based E. coli Topoisomerase IV DNA Decatenation Assay Kit Plus

GDDA100KE 100 assays
EUR 399.28
Description: This product includes all the reagents for 100 samples in gel-based assays of E. coli topoisomerase IV DNA decatenation activity. It includes 300 µl of 10 x Buffer, 205 µl of 60 mg/ml concatenated DNA (kDNA), 205 µl of 10 mM ATP, 550 µl of 5 x Gel loading buffer and 22 µl of 10 µM E.coli topoisomerase IV (the parC-parE complex).

96-well E. coli Topo IV DNA Cleavage Assay Kit Plus

T4C96KE 96 assays
EUR 597.7
Description: This product includes all the components in TDC96K plus 26 ul of 100 x E. coli Topo IV.

E. coli Topo IV DNA Relaxation Assay Kit Plus (enzyme included)

T4RA-100KE 100 assays
EUR 561.75
Description: This product includes 480 ul of 10 x Buffer, 405 ul of 10 x supercoiled plasmid DNA, 45 ul of 100 x E. coli topoisomerase IV, 420 ul of 10 mM ATP and 20 ul of 1500 x fluorescence dye H19 for 100 assays of DNA relaxation reactions in a 96-well assay format.

Deoxybrevianamide E

D083-1MG 1 mg
EUR 333.6

Deoxybrevianamide E

D083-5MG 5 mg
EUR 1076.4

Cytochalasin E

C197-1MG 1 mg
EUR 157.2

Cytochalasin E

C197-5MG 5 mg
EUR 462

Paraherquamide E

P070-0.5MG 0.5 mg
EUR 480

Paraherquamide E

P070-2.5MG 2.5 mg
EUR 1590

Phenelfamycin E

P078-1MG 1 mg
EUR 381.6

Phenelfamycin E

P078-5MG 5 mg
EUR 1248

Oligomycin E

O021-1MG 1 mg
EUR 381.6

Oligomycin E

O021-5MG 5 mg
EUR 1248

Roquefortine E

R035-1MG 1 mg
EUR 268.8

Roquefortine E

R035-5MG 5 mg
EUR 849.6

E. coli RNA polymerase Assay Kit Plus (E. coli RNA polymerase included)

RPA100KE 100 assays
EUR 467.15
Description: This product includes all the reagents in E. coli RNA Polymerase Assay Kit (Catalog number RPA100K) plus the enzyme, 33 ul of 100 x E. coli RNAP. It is for 100 assays of E. coli RNA polymerase reactions in a 384-well assay format.

Escherichia coli Serotype O2 (E. coli O2) Antibody

abx411330-02mg 0.2 mg
EUR 678

Escherichia coli Serotype O15 (E. coli O15) Antibody

abx411331-02mg 0.2 mg
EUR 678

Escherichia coli Serotype O20 (E. coli O20) Antibody

abx411332-02mg 0.2 mg
EUR 678

E. coli O157 antibody (FITC)

61C-CR1295M1F 100 ug
EUR 489.6
Description: Mouse monoclonal E. coli O157 antibody (FITC)

E. coli O157 antibody (FITC)

61C-CR1295M4F 100 ug
EUR 561.6
Description: Mouse monoclonal E. coli O157 antibody (FITC)

E. Coli Recombinant Thioredoxin 1

6329-50
EUR 483.6

E. coli O157:H7 antibody

70-XG13 1 mg
EUR 734.4
Description: Affinity purified Goat polyclonal E. coli O157:H7 antibody. 

E. coli antibody (K99 Pilus)

10-E20A 500 ug
EUR 591.6
Description: Mouse monoclonal E. coli antibody (K99 Pilus)

Insulin, human recombinant (E. coli)

4772-25
EUR 542.4

Insulin, human recombinant (E. coli)

4772-5
EUR 248.4

E. coli Tryptophanase (Tnase) Antibody

33517-05111 150 ug
EUR 313.2

E. coli Glycerol Kinase Antibody

32699-05111 150 ug
EUR 313.2

Transposition of Bordetella pertussis transposon in E. coli chromosome has been studied on a mannequin of exclusion of donor multicopy pKK3 plasmid with coumermicin. TnBP3 induced the formation of co-integrates between the plasmid and chromosome. The construction of co-integrate was decided. Info of exclusion of built-in construction and transposon transposition inside built-in plasmid into new websites on a recipient chromosome have been detected. Relationship between these processes and exercise of bacterial cell recombination system has been decided.

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