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Lenti vector viral particles from E. coli plasmids with Puro

A two-plasmid system for identification of promoters recognized by RNA polymerase containing extracytoplasmic stress response sigma(E) in Escherichia coli.

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A two-plasmid system for identification of promoters recognized by RNA polymerase containing extracytoplasmic stress response sigma(E) in Escherichia coli.

We have now beforehand established a two-plasmid system in Escherichia coli for identification of promoters acknowledged by RNA polymerase containing a heterologous sigma issue. Makes an attempt to optimize this method for identification of promoters acknowledged by RNA polymerase containing E. coli extracytoplasmic stress response sigma(E) failed owing to excessive toxicity of the expressed rpoE. A brand new system for identification of sigma(E)-cognate promoters was established, and verified utilizing the 2 recognized sigma(E)-dependent promoters, rpoEp2 and degPp. Expression of the sigma(E)-encoding rpoE gene was underneath the management of the AraC-dependent P(BAD) promoter.

A low stage of arabinose induced a non-toxic, nevertheless, ample stage of sigma(E) to work together with the core enzyme of RNA polymerase. Such an RNA polymerase holoenzyme acknowledged each recognized sigma(E)-dependent promoters, rpoEp2 and degPp, which have been cloned within the appropriate promoter probe plasmid, upstream of a promoterless lacZ alpha reporter gene. This new system has proved to be helpful for identification of E. coli sigma(E)-cognate promoters. Furthermore, the system could possibly be used for identification of ECF sigma-cognate promoters from different micro organism.

An built-in flow-injection processing (FIP) system for the quantification of plasmids throughout cultivation is described. The system performs on-line sampling, cell lysis, and quantification of plasmids in an built-in method throughout cultivation of E. coli. The system was operated through the use of a miniaturized expanded-bed column which can be utilized for dealing with samples containing cells and cell particles with out interfering with the binding evaluation. Two varieties of detectors (one measuring UV absorbance at 254 nm and a fluorometer) are used for on-line plasmid detection. The system was developed utilizing customary options and it was efficiently utilized in monitoring plasmid contents throughout a cultivation of E. coli.

Complementation between dimeric mutants as a probe of dimer-dimer interactions in tetrameric dihydrofolate reductase encoded by R67 plasmid of E. coli.

The impact of mutations on the interactions between dimers in R67 dihydrofolate reductase (R67 DHFR), a tetrameric enzyme conferring resistance to trimethoprim, was investigated by site-directed mutagenesis mixed with phenotypic, enzymatic, and biochemical evaluation. Some 14 mutants at two positions concerned in a hydrogen bond between dimers have been constructed. All have been proven to be dimers. Nevertheless, complementation between pairs of dimeric mutated proteins resulted within the restoration of the enzymatic exercise and heterotetramer formation. A combinatorial method was set as much as create effectively such heterotetramers and establish the complementing pairs of mutations. A dozen of such pairs have been discovered.

An correct methodology was set as much as measure the affiliation of the complementing dimers in a “quasi-isologous” heterotetramer and used to review the consequences of mutations and pH on the affiliation. Thus, the pair of proteins bearing respectively the S59A and H62L mutations was proven to type heterotetramers with catalytic properties near these of the wild-type protein. Its affiliation was as robust as that of the wild-type protein at cytoplasmic pH (6. 5), and was extra steady at decrease pH values.A double-mutant protein bearing concurrently the S59A and H62L mutations was produced and analyzed.

Its affiliation was weakened by 1.2 kcal/mol as in comparison with the wild-type enzyme at pH 6.5 however was insensitive to pH. Evaluating the vitality of affiliation between dimers within the wild-type protein, the heterotetramer and the double mutant allowed us to dissect the consequences of the pH and of the molecular context on a subset of interactions between the R67 DHFR subunits.

A two-plasmid system for identification of promoters recognized by RNA polymerase containing extracytoplasmic stress response sigma(E) in Escherichia coli.

Immunoadjuvant actions of E. coli– and plasmid-expressed recombinant hen IFN-alpha/beta, IFN-gamma and IL-1beta in 1-day- and 3-week-old chickens.

Within the current examine we assessed the capability of recombinant E. coli- or plasmid-expressed hen interferons (IFN) and hen IL-1beta, to exert immunostimulatory actions for humoral immune responses, in day-old and grownup chickens. We noticed that each recombinant E. coli-expressed hen IFN-alpha/beta and IFN-gamma facilitated the induction of a major and in addition a secondary antibody response, utilizing tetanus toxoid (TT) as a bacterial mannequin antigen, in immunologically mature 3-week-old chickens. In distinction, no enchancment of antibody both kind of hen IFN was co-injected with inactivated Infectious Bursal Illness Virus (IBDV) antigen.

TT-specific antibody formation was marginally elevated by co-injection of recombinant E. coli-expressed hen IL-1beta. Mixed administration of IFN-alpha/beta plus IFN-gamma or IL-beta elevated responses to TT in an additive, however not synergistic style. Remarkably, no augmentation of antibody responses particular for TT, nor IBDV, was famous in day-old birds, receiving IFN-alpha/beta or IFN-gamma as adjuvant. Additionally, intramuscular immunization of 3-week-old birds, utilizing plasmids encoding IFN-alpha/beta along with TT protein antigen, considerably elevated the pace and magnitude of TT-specific antibody responses. Plasmids encoding hen IL-beta or IFN-gamma had a minimal or inhibitory impact, respectively. These knowledge point out a possible for hen cytokines as immunoadjuvant for explicit varieties of hen vaccine antigens.

E. coli RecQ DNA helicase
01-003 50 ug
EUR 479
Description: The E. coli RecQ DNA helicase is available in Europe and for worldwide shipping via Gentaur.

E. coli RecQ DNA helicase
01-004 250? g
EUR 1549
Description: The E. coli RecQ DNA helicase is available in Europe and for worldwide shipping via Gentaur.

pBR322
G30-305 200µg
EUR 141

pBR322
G30-325 5x200µg
EUR 550

Uracil DNA Glycosylase Modified, from E. coli
GZ6540-500IU 500 IU
EUR 269

Uracil DNA Glycosylase Modified, from E. coli
GZ6540-5KIU 5 KIU
EUR 1107

T4 DNA Ligase, expressed in E. coli
GZ9900-1KIU 1 KIU
EUR 181

T4 DNA Ligase, expressed in E. coli
GZ9900-5KIU 5 KIU
EUR 469

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N150 500 units
EUR 102

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N152 5x500 units
EUR 394

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N154 20x500 units
EUR 1525

Escherichia coli (E. coli) Antibody
abx411320-1ml 1 ml
EUR 509

Escherichia coli (E. coli) Antibody
abx415692-01mg 0.1 mg
EUR 634

Escherichia coli (E. coli) Antibody
abx415712-1ml 1 ml
EUR 606

E. coli antibody
20C-CR1244GP 1 ml
EUR 468
Description: Goat polyclonal E. coli antibody

E. coli antibody
10C-CR1244M1 100 ug
EUR 408
Description: Mouse monoclonal E. coli antibody

E. coli antibody
10-E09C 100 ug
EUR 133
Description: Mouse monoclonal E. coli antibody

E. coli antibody
10-E10A 500 ug
EUR 493
Description: Mouse monoclonal E. coli antibody

E. coli antibody
10-E10B 500 ug
EUR 493
Description: Mouse monoclonal E. coli antibody

E. coli antibody
20-ER13 1 ml
EUR 355
Description: Rabbit polyclonal E. coli antibody

E. coli GroL
DAG1704 100 ug
EUR 915

E. coli GroS
DAG1705 100 ug
EUR 915

E. coli DsbA
DAG1712 100 ug
EUR 915

E. coli GrpE
DAG1714 100 ug
EUR 1027

E. coli Antibody
abx021669-1mg 1 mg
EUR 1295

E. coli Antibody
abx021670-1mg 1 mg
EUR 871

E. coli Antibody
abx021671-02ml 0.2 ml
EUR 495

E. coli Antibody
abx021672-1mg 1 mg
EUR 871

E. coli Antibody
abx021673-1mg 1 mg
EUR 871

E. coli Antibody
abx022970-1ml 1 ml
EUR 732

E. coli Antibody
abx022971-1ml 1 ml
EUR 968

E. coli Antibody
abx022975-1ml 1 ml
EUR 599

E. coli Antibody
abx022977-1ml 1 ml
EUR 676

E. coli DNA Polymerase I (Pol I) - 2,500 units
3268 1/EA
EUR 305

Crude Extracellular Mixture of E. coli, DNA Aptamer, Biotinylated
AD-112-B Custom Ask for price

Crude Extracellular Mixture of E. coli, DNA Aptamer, unlabeled
AD-112-U Custom Ask for price

Escherichia coli (E. coli) Antibody (Biotin)
abx411321-1ml 1 ml
EUR 592

Escherichia coli (E. coli) Antibody (FITC)
abx411322-1ml 1 ml
EUR 509

Escherichia coli (E. coli) Antibody (Biotin)
abx415713-1ml 1 ml
EUR 718

100 BP DNA LADDER, 500UL PER KIT
M-DNA-100BP 1/pk
EUR 73
Description: Bioscience Mol Bio; DNA Ladder

1 KB DNA LADDER, 500UL PER KIT
M-DNA-1KB 1/pk
EUR 70
Description: Bioscience Mol Bio; DNA Ladder

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (50ug Relaxed DNA + 100u Gyrase)
TG2000G-1KIT 100 assays
EUR 380

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (250ug Relaxed DNA + 500u Gyrase)
TG2000G-3KIT 500 assays
EUR 985

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (500ug Relaxed DNA + 1000u Gyrase)
TG2000G-5KIT 1000 assays
EUR 1612

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (1mg Relaxed DNA + 2000u Gyrase)
TG2000G-7KIT 2000 assays
EUR 2844

Purified E. coli DNA Gyrase Drug Screening Kit (50ug Relaxed DNA + 100u Gyrase)
TG2001G-1KIT 100 assays
EUR 414

Purified E. coli DNA Gyrase Drug Screening Kit (250ug Relaxed DNA + 500u Gyrase)
TG2001G-3KIT 500 assays
EUR 1052

Purified E. coli DNA Gyrase Drug Screening Kit (500ug Relaxed DNA + 1000u Gyrase)
TG2001G-5KIT 1000 assays
EUR 1836

Purified E. coli DNA Gyrase Drug Screening Kit (1mg Relaxed DNA + 2000u Gyrase)
TG2001G-7KIT 2000 assays
EUR 2956

E. coli Topoisomerase IV Drug Screening Kit (25ug SC DNA)
TG1007-1 100 assays
EUR 324

E. coli Topoisomerase IV Drug Screening Kit (62.5ug SC DNA)
TG1007-2 250 assays
EUR 448

Crude Extracellular Mixture of E. coli, DNA Aptamer, FITC labeled
AD-112-F Custom Ask for price

Benzonase, E. Coli Recombinant
7680-25
EUR 332

Benzonase, E. Coli Recombinant
7680-5
EUR 137

E. Coli HCP antibody
20R-EG001 1 ml
EUR 837
Description: Goat polyclonal E. Coli HCP antibody

E. coli O157 antibody
10C-CR1295M3 100 ug
EUR 392
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody
10-1490 100 ug
EUR 136
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody
10-1521 100 ug
EUR 142
Description: Mouse monoclonal E. coli O157 antibody

E. coli O157 antibody
10-E12A 100 ug
EUR 385
Description: Mouse monoclonal E. coli O157 antibody

TesA, E. coli esterase
0306-1 100 Units
EUR 421

TesA, E. coli esterase
0306-5 500 Units
EUR 1705

E. Coli Recombinant TRXB
6331-100
EUR 425

E. Coli Recombinant SlyD
6366-100
EUR 457

E. coli antibody (FITC)
60-E13 1 ml
EUR 378
Description: Rabbit polyclonal E. coli antibody (FITC) conjugated

E. coli antibody (biotin)
60-E13B 1 ml
EUR 468
Description: Rabbit polyclonal E. coli antibody (biotin) conjugated

E. coli antibody (HRP)
60-E14 1 ml
EUR 408
Description: Rabbit polyclonal E. coli antibody (HRP) conjugated

E. coli Recombinant IDNK
P1050-10
EUR 164

E. coli Recombinant IDNK
P1050-50
EUR 381

Recombinant E. coli, Glucokinase
P1054-10
EUR 164

Recombinant E. coli, Glucokinase
P1054-50
EUR 381

E. coli Exonuclease I
M1222-100
EUR 251

E. coli Exonuclease III
M1223-100
EUR 267

RecA Protein E. coli
M1231-200
EUR 202

E. coli FimC [His]
DAG1666 100 ug
EUR 1027

E. coli Eco [His]
DAG1667 50 ug
EUR 1027

E. coli O157 Antibody
abx021684-1mg 1 mg
EUR 1017

E. coli O157 Antibody
abx021685-1mg 1 mg
EUR 996

E. coli O157 Antibody
abx021686-1mg 1 mg
EUR 996

E. coli O157 Antibody
abx021687-1mg 1 mg
EUR 996

E. coli O157 Antibody
abx021688-1mg 1 mg
EUR 940

E. coli O157 Antibody
abx021689-1mg 1 mg
EUR 996

E. coli Antibody (HRP)
abx022972-1ml 1 ml
EUR 599

E. coli Antibody (FITC)
abx022974-1ml 1 ml
EUR 648

E. coli Antibody (Biotin)
abx022976-1ml 1 ml
EUR 599

E. coli Antibody (HRP)
abx022978-1ml 1 ml
EUR 878

E. coli Antibody (Biotin)
abx022979-1ml 1 ml
EUR 739

E. coli O157 Antibody
abx024017-1mg 1 mg
EUR 1010

E. coli O 157
abx291002-100mg 100 mg
EUR 620

E. coli Topoisomerase IV
TG2000EC-1 250 units
EUR 448

E. coli Topoisomerase IV
TG2000EC-3 500 units
EUR 683

E. coli Topoisomerase IV
TG2000EC-5 1000 units
EUR 1052

E. coli GroEL Antibody
20012-05011 150 ug
EUR 217

E. coli CysH Antibody
30281-05111 150 ug
EUR 261

E. coli DnaJ Antibody
32022-05111 150 ug
EUR 261

E. coli pykF Antibody
32163-05111 150 ug
EUR 261

E. coli GOR Antibody
32540-05111 150 ug
EUR 261

E. coli NDK Antibody
32662-05111 150 ug
EUR 261

E. coli PPA Antibody
32678-05111 150 ug
EUR 261

E. coli glk Antibody
32864-05111 150 ug
EUR 261

E. Coli ldhA Antibody
32898-05111 150 ug
EUR 261

E. coli deoC Antibody
32936-05111 150 ug
EUR 261

E. coli Skp Antibody
32971-05111 150 ug
EUR 261

E. coli nanA Antibody
33092-05111 150 ug
EUR 261

E. coli RecA Protein
01-001 200 ug
EUR 205
Description: The E. coli RecA Protein is available in Europe and for worldwide shipping via Gentaur.

E. coli LexA repressor
01-005 50 ug
EUR 479
Description: The E. coli LexA repressor is available in Europe and for worldwide shipping via Gentaur.

E. coli LexA repressor
01-006 250 ug
EUR 1184
Description: The E. coli LexA repressor is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvA protein
01-007 50 ug
EUR 479
Description: The E. coli RuvA protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvA protein
01-008 250 ug
EUR 1184
Description: The E. coli RuvA protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvB protein
01-009 50 ug
EUR 479
Description: The E. coli RuvB protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvB protein
01-010 250 ug
EUR 1184
Description: The E. coli RuvB protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvC protein
01-011 50 ug
EUR 479
Description: The E. coli RuvC protein is available in Europe and for worldwide shipping via Gentaur.

E. coli RuvC protein
01-012 250 ug
EUR 1184
Description: The E. coli RuvC protein is available in Europe and for worldwide shipping via Gentaur.

Enterotoxin STp (E. coli)
H-6248.0500 0.5mg
EUR 696
Description: Sum Formula: C81H110N20O26S6; CAS# [115474-04-9] net

Enterotoxin STp (E. coli)
H-6248.1000 1.0mg
EUR 1180
Description: Sum Formula: C81H110N20O26S6; CAS# [115474-04-9] net

Human Escherichia coli (E. coli) ELISA Kit
abx052493-96tests 96 tests
EUR 668

Cow Escherichia coli (E. coli) ELISA Kit
abx055785-96tests 96 tests
EUR 668

E. Coli Proteins-Agarose affinity gel for removing E. coli antibodies
EC11-G 1 ml
EUR 286

Escherichia coli Serotype O2 (E. coli O2) Antibody
abx411330-02mg 0.2 mg
EUR 565

Escherichia coli Serotype O15 (E. coli O15) Antibody
abx411331-02mg 0.2 mg
EUR 565

Escherichia coli Serotype O20 (E. coli O20) Antibody
abx411332-02mg 0.2 mg
EUR 565

rHu BMP-2 (E. coli)
AK8356-0002 2µg Ask for price

rHu BMP-2 (E. coli)
AK8356-0010 10µg Ask for price

rHu BMP-2 (E. coli)
AK8356-0100 100µg Ask for price

rHu BMP-2 (E. coli)
AK8356-1000 1mg Ask for price

E. coli O157:H7 antibody
70-XG13 1 mg
EUR 612
Description: Affinity purified Goat polyclonal E. coli O157:H7 antibody. 

Insulin, human recombinant (E. coli)
4772-25
EUR 452

Insulin, human recombinant (E. coli)
4772-5
EUR 207

E. coli antibody (K99 Pilus)
10-E20A 500 ug
EUR 493
Description: Mouse monoclonal E. coli antibody (K99 Pilus)

E. coli O157 antibody (FITC)
61C-CR1295M1F 100 ug
EUR 408
Description: Mouse monoclonal E. coli O157 antibody (FITC)

E. coli O157 antibody (FITC)
61C-CR1295M4F 100 ug
EUR 468
Description: Mouse monoclonal E. coli O157 antibody (FITC)

E. Coli Recombinant Thioredoxin 1
6329-50
EUR 403

E. coli Recombinant Carbonic anhydrase
P1049-10
EUR 164

E. coli Recombinant Carbonic anhydrase
P1049-50
EUR 381

E. coli β-gal peptide
DAG2441 0.05 mg
EUR 485

Native E. Coli O 158
DAGA-3025 0.1 ml
EUR 715

Uricase from E. coli, Recombinant
DIA-415 5KU
EUR 378

Recombinant Diacylglycerol Kinase (E. coli)
MET-5035 100 mg
EUR 276
Description: Purified recombinant prokaryotic DAG kinase expressed in E. coli

Recombinant Diacylglycerol Kinase (E. coli)
MET-5035-5 5 x 100 mg
EUR 920
Description: Purified recombinant prokaryotic DAG kinase expressed in E. coli

E. Coli O 157 Protein
abx061475-01ml 0.1 ml
EUR 551

E. coli Enterotoxin B Antibody
abx021678-1mg 1 mg
EUR 599

E. coli Enterotoxin B Antibody
abx021679-1mg 1 mg
EUR 606

E. coli Enterotoxin B Antibody
abx021680-1mg 1 mg
EUR 599

E. coli O157 Antibody (FITC)
abx021683-1mg 1 mg
EUR 2332

E. coli O157:H7 Antibody
abx022980-1mg 1 mg
EUR 599

E. coli Enterotoxin LT Antibody
abx023834-1mg 1 mg
EUR 732

E. coli Enterotoxin LT Antibody
abx023835-1mg 1 mg
EUR 732

E. coli Enterotoxin LT Antibody
abx023836-1mg 1 mg
EUR 732

E. coli Enterotoxin LT Antibody
abx023837-1mg 1 mg
EUR 732

E. coli Enterotoxin STp Antibody
abx023838-1mg 1 mg
EUR 878

E. coli Enterotoxin STp Antibody
abx023839-1mg 1 mg
EUR 878

E. coli Enterotoxin STp Antibody
abx023840-1mg 1 mg
EUR 878

E. coli O157 (9.88) Antibody
BNCA0207-250 250uL
EUR 383
Description: Primary antibody against E. coli O157 (9.88),APC conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNCAP0207-100 100uL
EUR 199
Description: Primary antibody against E. coli O157 (9.88),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNCAP0207-500 500uL
EUR 544
Description: Primary antibody against E. coli O157 (9.88),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC810207-100 100uL
EUR 199
Description: Primary antibody against E. coli O157 (9.88),CF680R conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC810207-500 500uL
EUR 544
Description: Primary antibody against E. coli O157 (9.88),CF680R conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC880207-100 100uL
EUR 199
Description: Primary antibody against E. coli O157 (9.88),CF488A conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC880207-500 500uL
EUR 544
Description: Primary antibody against E. coli O157 (9.88),CF488A conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNCB0207-100 100uL
EUR 199
Description: Primary antibody against E. coli O157 (9.88),Biotin conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNCB0207-500 500uL
EUR 544
Description: Primary antibody against E. coli O157 (9.88),Biotin conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC550207-100 100uL
EUR 199
Description: Primary antibody against E. coli O157 (9.88),CF555 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC550207-500 500uL
EUR 544
Description: Primary antibody against E. coli O157 (9.88),CF555 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC470207-100 100uL
EUR 199
Description: Primary antibody against E. coli O157 (9.88),CF647 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC470207-500 500uL
EUR 544
Description: Primary antibody against E. coli O157 (9.88),CF647 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC940207-100 100uL
EUR 199
Description: Primary antibody against E. coli O157 (9.88),CF594 conjugate, Concentration: 0.1mg/mL

E. coli O157 (9.88) Antibody
BNC940207-500 500uL
EUR 544
Description: Primary antibody against E. coli O157 (9.88),CF594 conjugate, Concentration: 0.1mg/mL

Transposition of Bordetella pertussis transposon in E. coli chromosome has been studied on a mannequin of exclusion of donor multicopy pKK3 plasmid with coumermicin. TnBP3 induced the formation of co-integrates between the plasmid and chromosome. The construction of co-integrate was decided. Info of exclusion of built-in construction and transposon transposition inside built-in plasmid into new websites on a recipient chromosome have been detected. Relationship between these processes and exercise of bacterial cell recombination system has been decided.

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