BioConstructor Molecular Cloning Software

Lenti vector viral particles from E. coli plasmids with Puro

A two-plasmid system for identification of promoters recognized by RNA polymerase containing extracytoplasmic stress response sigma(E) in Escherichia coli.

Posted on by Tiffany
A two-plasmid system for identification of promoters recognized by RNA polymerase containing extracytoplasmic stress response sigma(E) in Escherichia coli.

We have now beforehand established a two-plasmid system in Escherichia coli for identification of promoters acknowledged by RNA polymerase containing a heterologous sigma issue. Makes an attempt to optimize this method for identification of promoters acknowledged by RNA polymerase containing E. coli extracytoplasmic stress response sigma(E) failed owing to excessive toxicity of the expressed rpoE. A brand new system for identification of sigma(E)-cognate promoters was established, and verified utilizing the 2 recognized sigma(E)-dependent promoters, rpoEp2 and degPp. Expression of the sigma(E)-encoding rpoE gene was underneath the management of the AraC-dependent P(BAD) promoter.

A low stage of arabinose induced a non-toxic, nevertheless, ample stage of sigma(E) to work together with the core enzyme of RNA polymerase. Such an RNA polymerase holoenzyme acknowledged each recognized sigma(E)-dependent promoters, rpoEp2 and degPp, which have been cloned within the appropriate promoter probe plasmid, upstream of a promoterless lacZ alpha reporter gene. This new system has proved to be helpful for identification of E. coli sigma(E)-cognate promoters. Furthermore, the system could possibly be used for identification of ECF sigma-cognate promoters from different micro organism.

An built-in flow-injection processing (FIP) system for the quantification of plasmids throughout cultivation is described. The system performs on-line sampling, cell lysis, and quantification of plasmids in an built-in method throughout cultivation of E. coli. The system was operated through the use of a miniaturized expanded-bed column which can be utilized for dealing with samples containing cells and cell particles with out interfering with the binding evaluation. Two varieties of detectors (one measuring UV absorbance at 254 nm and a fluorometer) are used for on-line plasmid detection. The system was developed utilizing customary options and it was efficiently utilized in monitoring plasmid contents throughout a cultivation of E. coli.

Complementation between dimeric mutants as a probe of dimer-dimer interactions in tetrameric dihydrofolate reductase encoded by R67 plasmid of E. coli.

The impact of mutations on the interactions between dimers in R67 dihydrofolate reductase (R67 DHFR), a tetrameric enzyme conferring resistance to trimethoprim, was investigated by site-directed mutagenesis mixed with phenotypic, enzymatic, and biochemical evaluation. Some 14 mutants at two positions concerned in a hydrogen bond between dimers have been constructed. All have been proven to be dimers. Nevertheless, complementation between pairs of dimeric mutated proteins resulted within the restoration of the enzymatic exercise and heterotetramer formation. A combinatorial method was set as much as create effectively such heterotetramers and establish the complementing pairs of mutations. A dozen of such pairs have been discovered.

An correct methodology was set as much as measure the affiliation of the complementing dimers in a “quasi-isologous” heterotetramer and used to review the consequences of mutations and pH on the affiliation. Thus, the pair of proteins bearing respectively the S59A and H62L mutations was proven to type heterotetramers with catalytic properties near these of the wild-type protein. Its affiliation was as robust as that of the wild-type protein at cytoplasmic pH (6. 5), and was extra steady at decrease pH values.A double-mutant protein bearing concurrently the S59A and H62L mutations was produced and analyzed.

Its affiliation was weakened by 1.2 kcal/mol as in comparison with the wild-type enzyme at pH 6.5 however was insensitive to pH. Evaluating the vitality of affiliation between dimers within the wild-type protein, the heterotetramer and the double mutant allowed us to dissect the consequences of the pH and of the molecular context on a subset of interactions between the R67 DHFR subunits.

A two-plasmid system for identification of promoters recognized by RNA polymerase containing extracytoplasmic stress response sigma(E) in Escherichia coli.

Immunoadjuvant actions of E. coli– and plasmid-expressed recombinant hen IFN-alpha/beta, IFN-gamma and IL-1beta in 1-day- and 3-week-old chickens.

Within the current examine we assessed the capability of recombinant E. coli- or plasmid-expressed hen interferons (IFN) and hen IL-1beta, to exert immunostimulatory actions for humoral immune responses, in day-old and grownup chickens. We noticed that each recombinant E. coli-expressed hen IFN-alpha/beta and IFN-gamma facilitated the induction of a major and in addition a secondary antibody response, utilizing tetanus toxoid (TT) as a bacterial mannequin antigen, in immunologically mature 3-week-old chickens. In distinction, no enchancment of antibody both kind of hen IFN was co-injected with inactivated Infectious Bursal Illness Virus (IBDV) antigen.

TT-specific antibody formation was marginally elevated by co-injection of recombinant E. coli-expressed hen IL-1beta. Mixed administration of IFN-alpha/beta plus IFN-gamma or IL-beta elevated responses to TT in an additive, however not synergistic style. Remarkably, no augmentation of antibody responses particular for TT, nor IBDV, was famous in day-old birds, receiving IFN-alpha/beta or IFN-gamma as adjuvant. Additionally, intramuscular immunization of 3-week-old birds, utilizing plasmids encoding IFN-alpha/beta along with TT protein antigen, considerably elevated the pace and magnitude of TT-specific antibody responses. Plasmids encoding hen IL-beta or IFN-gamma had a minimal or inhibitory impact, respectively. These knowledge point out a possible for hen cytokines as immunoadjuvant for explicit varieties of hen vaccine antigens.

E. coli DNA Ligase
M1217-100 each
EUR 385.2

E. coli DNA Gyrase
TG2000G-1 100 units
EUR 388.8

E. coli DNA Gyrase
TG2000G-3 500 units
EUR 846

E. coli DNA Gyrase
TG2000G-5 1000 units
EUR 1396.8

E. coli DNA Gyrase
TG2000G-7 2000 units
EUR 2203.2

E. coli RecQ DNA helicase
01-003 50 ug
EUR 574.8
Description: The E. coli RecQ DNA helicase is available in Europe and for worldwide shipping via Gentaur.

E. coli RecQ DNA helicase
01-004 250? g
EUR 1858.8
Description: The E. coli RecQ DNA helicase is available in Europe and for worldwide shipping via Gentaur.

E. coli DNA Polymerase I
M1210-100 each
EUR 307.2

Escherichia coli Plasmid-derived single-stranded DNA-binding protein (ssbF)
1-CSB-EP323579ENV
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Plasmid-derived single-stranded DNA-binding protein(ssbF) expressed in E.coli

E. coli DNA polymerase Assay Kit Plus (E. coli DNA pol III alpha subunit included)
DPA100KE 100 assays
EUR 444.91
Description: This product includes all the reagents in the DNA Polymerase III Alpha Assay Kit (Catalog No. DPA100K) plus 35 ul of 100 x E. coli DNA polymerase III alpha subunit.

E. coli DNA Primase Assay Kit Plus (E. coli primase-helicase included)
EGA100KE 100 assays
EUR 444.91
Description: This product includes all the kit components in E. coli DNA Primase Assay Kit (Catalog No. EGA100K ) plus 45 ul of 100 x E. coli primase-helicase complex. It is for 100 assays of E.coli DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format.

E. coli DNA ligase - for 100 assays
LIG-100EC 100 assays
EUR 309.4
Description: This product includes 10 ul of 500 nM E. coli ligase.

E. coli DNA gyrase - for 100 assays
TOP2-100EC 100 assays
EUR 309.4
Description: This product includes wild-type E. coli gyrase for 100 assays (40 ul of 2000 nM stock solution)

E. coli DNA primase - for 100 assays
DNAG-100EC 100 assays
EUR 309.4
Description: This product includes 45 ul of 10 uM E. coli primase-helicase complex

E. coli DNA helicase - for 100 assays
DNAB-100EC 100 assays
EUR 309.4
Description: This product includes 33 ul of 20 uM E. coli DNA helicase.

T4 DNA Ligase, expressed in E. coli
GZ9900-1KIU 1 KIU
EUR 217.2

T4 DNA Ligase, expressed in E. coli
GZ9900-5KIU 5 KIU
EUR 562.8

E. coli DNA Topoisomerase I Assay Kit Plus (E. coli Topo I included)
DRA020KE 20 assays
EUR 221.78
Description: This product includes 160 ul of 10 x Buffer T1, 84 ul of 10 x supercoiled plasmid DNA, 10 ul of 100 x E. coli topoisomerase I, 8 ul of 1500 x Dye H19 and 500 ul of 10 x H19 dilution buffer. It is for 20 DNA relaxation assays in a 96-well plate format or 40 assays in a 384-well assay format.

E. coli DNA Topoisomerase IV Assay Kit Plus (E. coli Topo IV included)
DDC020KE 20 assays
EUR 221.78
Description: This product includes all the components in DNA Topoisomerase IV Assay Kit (Catalog No. DDC020K)plus 12ul of 100 x E.coli topoisomerase IV (500 nM) for 20 E. coli topoisomerase IV DNA decatenation assays.

E. coli NAD+-dependent DNA ligase assay kit Plus (E. coli ligase included)
NLA100KE 100 assays
EUR 444.91
Description: This product includes 800 ul of 10 x buffer, 44 ul of 100x DNA substrate, 44 ul of 100 x NAD+, 21 ml of Reagent T, 10 ul of 500 x E. coli ligase and 220 ul of 10 x fluorescence dye for 100 assays.

E. coli DNA Topoisomerase II (Gyrase) Assay Kit Plus (E. coli gyrase included)
DSA020KE 20 assays
EUR 221.78
Description: This product includes160 ul of 10 x Buffer T2, 84 ul of 10 x relaxed DNA, 6 ul of 1500 x H19 dye, 90 ul of 10 x ATP and 550 ul of 10 x H19 dilution buffer. It is for 20 assays in 96-well plate format. E. coli gyrase is not included.

Uracil DNA Glycosylase Modified, from E. coli
GZ6540-500IU 500 IU
EUR 322.8

Uracil DNA Glycosylase Modified, from E. coli
GZ6540-5KIU 5 KIU
EUR 1328.4

T4 DNA Ligase Plasmid
PVT7008 2 ug
EUR 614.4

E. coli DNA polymerase alpha - for 100 assays
DPA-100EC 100 assays
EUR 309.4
Description: This product includes 33 ul of 2 uM E. coli DNA polymerase III alpha.

E. coli DNA topoisomerase I - for 100 assays
TOP1-100EC 100 assays
EUR 309.4
Description: This product includes 42 ul of 4 uM E. coli topoisomerase I

ApexPrep DNA Plasmid Miniprep Column Only
A5002-300 300 tests
EUR 296.4

ApexPrep DNA Plasmid Miniprep Column Only
A5002-50 50 tests
EUR 104.4

Relaxed plasmid DNA - 0.050 mg
RDNA-050UG 0.050 mg
EUR 181.67
Description: This product includes 0.050 mg of relaxed plasmid DNA at 1 mg/ml in TE buffer

Supercoiled plasmid DNA - 0.050 mg
SDNA-050UG 0.050 mg
EUR 160.91
Description: This product includes 0.050 mg of supercoiled plasmid DNA at 1 mg/ml in TE buffer

ApexPrep DNA Plasmid Miniprep Kit
A5001-300 300 tests
EUR 338.4

ApexPrep DNA Plasmid Miniprep Kit
A5001-50 50 tests
EUR 115.2

Relaxed plasmid DNA - 1 mg
RDNA-1MG 1 mg
EUR 2298.23
Description: This product includes 1 mg of relaxed plasmid DNA at 1 mg/ml in TE buffer

Plasmid DNA Maxiprep (10 preps)
9K-006-0023 10Preps
EUR 257.32

Plasmid DNA Maxiprep (30 preps)
9K-006-0026 30preps
EUR 651.95

Supercoiled Plasmid DNA -1 mg
SDNA-1MG 1 mg
EUR 1752.66
Description: This product includes 1 mg of supercoiled plasmid DNA at 1 mg/ml concentration in TE buffer.

Recombinant Plasmodium Falciparum DNA-directed RNA polymerase III subunit RPC1 Protein (E. coli)
VAng-Wyb6412-inquire inquire Ask for price
Description: Plasmodium Falciparum DNA-directed RNA polymerase III subunit RPC1, partial, recombinant protein.

EZ-10 Spin Column Plasmid DNA Miniprep Kit, Low Copy Plasmid
BS4139 50Preps
EUR 105.67

EZ-10 Spin Column Plasmid DNA Miniprep Kit, Low Copy Plasmid
BS4149 100preps
EUR 136.74

E. coli DNA Helicase ATPase assay Kit Plus-100
DNAB100KE 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x E. coli DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

E. coli DNA Helicase ATPase assay Kit Plus-500
DNAB500KE 500 assays
EUR 1396.74
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x E. coli DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

E. coli DNA Primase Assay Kit (enzyme not included)
EGA100K 100 assays
EUR 278.4
Description: This product includes 600 ul of 10 x assay buffer, 45 ul of 100 x DNA template, 45 ul of 100 x NTP mix and 850 ul of 10 x fluorescence dye for 100 assays of E. coli DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format. The kit does not include the enzyme.

E. coli single-strand DNA binding protein - 100 ug
SSB-100EC 100 ug
EUR 234.99
Description: This product includes 100 ug of E. coli single strand DNA binding protein

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (1mg Relaxed DNA + 2000u Gyrase)
TG2000G-7KIT 2000 assays
EUR 3412.8

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (500ug Relaxed DNA + 1000u Gyrase)
TG2000G-5KIT 1000 assays
EUR 1934.4

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (50ug Relaxed DNA + 100u Gyrase)
TG2000G-1KIT 100 assays
EUR 456

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (250ug Relaxed DNA + 500u Gyrase)
TG2000G-3KIT 500 assays
EUR 1182

EZ-10 Spin Column Plasmid DNA Min-preps Kit, Low Copy Plasmid
BS6149 250preps
EUR 251.05

Purified E. coli DNA Gyrase Drug Screening Kit (1mg Relaxed DNA + 2000u Gyrase)
TG2001G-7KIT 2000 assays
EUR 3547.2

Crude Extracellular Mixture of E. coli, DNA Aptamer, unlabeled
AD-112-U Custom Ask for price

Purified E. coli DNA Gyrase Drug Screening Kit (500ug Relaxed DNA + 1000u Gyrase)
TG2001G-5KIT 1000 assays
EUR 2203.2

Plasmid DNA Extraction and Purification
EP10014 100 Tests
EUR 403.2

E. coli DnaJ Antibody
32022-05111 150 ug
EUR 313.2

Crude Extracellular Mixture of E. coli, DNA Aptamer, Biotinylated
AD-112-B Custom Ask for price

Purified E. coli DNA Gyrase Drug Screening Kit (50ug Relaxed DNA + 100u Gyrase)
TG2001G-1KIT 100 assays
EUR 496.8

Purified E. coli DNA Gyrase Drug Screening Kit (250ug Relaxed DNA + 500u Gyrase)
TG2001G-3KIT 500 assays
EUR 1262.4

HLA-E cloning plasmid
CSB-CL320269HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the HLA-E gene.

EZ-10 Spin Column Plasmid DNA Miniprep Kit
BS413 50Preps
EUR 91.97

EZ-10 Spin Column Plasmid DNA Miniprep Kit
BS414 100preps
EUR 114.82

Escherichia coli (E. coli) Antibody
abx415692-01mg 0.1 mg
EUR 760.8

Escherichia coli (E. coli) Antibody
abx415712-1ml 1 ml
EUR 727.2

Escherichia coli (E. coli) Antibody
abx411320-1ml 1 ml
EUR 610.8

pDONR223-HLA-E Plasmid
PVTB00773 2 ug
EUR 427.2

E. coli Topoisomerase IV Drug Screening Kit (62.5ug SC DNA)
TG1007-2 250 assays
EUR 537.6

Plasmid DNA Extraction Mini Kit (100prep)
FAPDE-001 100 preps
EUR 168

Plasmid DNA Extraction Mini Kit (300prep)
FAPDE-001-1 300 preps
EUR 241.2

Plasmid DNA Extraction Mini Kit (100prep)
FAPDE-100 100 preps
EUR 154.8

Plasmid DNA Extraction Mini Kit (300prep)
FAPDE-300 300 preps
EUR 206.4

E. coli Topoisomerase IV Drug Screening Kit (25ug SC DNA)
TG1007-1 100 assays
EUR 388.8

E. coli GroL
DAG1704 100 ug
EUR 1098

E. coli GroS
DAG1705 100 ug
EUR 1098

E. coli DsbA
DAG1712 100 ug
EUR 1098

E. coli GrpE
DAG1714 100 ug
EUR 1232.4

Gel-Based E. coli Topoisomerase I DNA relaxation Assay Kit
GDRA100KE 100 assays
EUR 399.28
Description: This product includes all the reagents for 100 samples in gel-based assays of E. coli topo I DNA relaxation activity. It This product includes 300 µl of 10 x Buffer T1, 100 µl of 250 mg/ml supercoiled DNA, 550 µl of 5 x Gel loading buffer and 10 µl of 4 µM E. coli topoisomerase I enzyme.

Recombinant Plasmodium Berghei CS Protein (E. coli)
VAng-Wyb7615-inquire inquire Ask for price
Description: Plasmodium Berghei (strain Anka) Circumsporozoite protein, partial, recombinant protein.

EZ-10 Spin Column Plasmid DNA Min-preps Kit
BS614 250preps
EUR 199.9

Plasmid DNA extraction and purification kit
K1445-100 each
EUR 444

E. coli antibody
10C-CR1244M1 100 ug
EUR 489.6
Description: Mouse monoclonal E. coli antibody

E. coli antibody
20-ER13 1 ml
EUR 426
Description: Rabbit polyclonal E. coli antibody

E. coli antibody
10-E09C 100 ug
EUR 159.6
Description: Mouse monoclonal E. coli antibody

E. coli antibody
10-E10A 500 ug
EUR 591.6
Description: Mouse monoclonal E. coli antibody

E. coli antibody
10-E10B 500 ug
EUR 591.6
Description: Mouse monoclonal E. coli antibody

E. coli antibody
20C-CR1244GP 1 ml
EUR 561.6
Description: Goat polyclonal E. coli antibody

E. coli Antibody
abx022970-1ml 1 ml
EUR 878.4

E. coli Antibody
abx022971-1ml 1 ml
EUR 1161.6

E. coli Antibody
abx022975-1ml 1 ml
EUR 718.8

E. coli Antibody
abx022977-1ml 1 ml
EUR 811.2

E. coli Antibody
abx021669-1mg 1 mg
EUR 1554

E. coli Antibody
abx021670-1mg 1 mg
EUR 1045.2

E. coli Antibody
abx021671-02ml 0.2 ml
EUR 594

E. coli Antibody
abx021672-1mg 1 mg
EUR 1045.2

E. coli Antibody
abx021673-1mg 1 mg
EUR 1045.2

Recombinant Plasmodium Berghei COI Protein (E. coli)
VAng-Wyb7599-inquire inquire Ask for price
Description: Plasmodium Berghei Cytochrome c oxidase subunit 1 protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii CPK1 Protein (E. coli)
VAng-Wyb7894-inquire inquire Ask for price
Description: Plasmodium Yoelii Calcium-dependent protein kinase 1 protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii CPK3 Protein (E. coli)
VAng-Wyb7898-inquire inquire Ask for price
Description: Plasmodium Yoelii Calcium-dependent protein kinase 3 protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii CPK4 Protein (E. coli)
VAng-Wyb7902-inquire inquire Ask for price
Description: Plasmodium Yoelii Calcium-dependent protein kinase 4 protein, partial, recombinant protein.

Crude Extracellular Mixture of E. coli, DNA Aptamer, FITC labeled
AD-112-F Custom Ask for price

Recombinant Plasmodium Berghei CG10 Protein (E. coli)
VAng-Wyb7595-inquire inquire Ask for price
Description: Plasmodium Berghei Putative chloroquine resistance transporter protein, partial, recombinant protein.

Recombinant Plasmodium Berghei CPK4 Protein (E. coli)
VAng-Wyb7603-inquire inquire Ask for price
Description: Plasmodium Berghei (strain Anka) Calcium-dependent protein kinase 4 protein, partial, recombinant protein.

Recombinant Plasmodium Chabaudi COI Protein (E. coli)
VAng-Wyb7711-inquire inquire Ask for price
Description: Plasmodium Chabaudi Cytochrome c oxidase subunit 1 protein, partial, recombinant protein.

Recombinant Plasmodium Chabaudi CG10 Protein (E. coli)
VAng-Wyb7707-inquire inquire Ask for price
Description: Plasmodium Chabaudi Putative chloroquine resistance transporter protein, partial, recombinant protein.

EZ-500 Spin Column Plasmid DNA Maxi-preps Kit
BS4654 4preps
EUR 150.92

EZ-500 Spin Column Plasmid DNA Maxi-preps Kit
BS466 20Preps
EUR 466.63

Recombinant Plasmodium Yoelii PY01232 Protein (E. coli)
VAng-Wyb7946-inquire inquire Ask for price
Description: Plasmodium Yoelii Myosin-A protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii PY03426 Protein (E. coli)
VAng-Wyb7978-inquire inquire Ask for price
Description: Plasmodium Yoelii Translation factor GUF1 homolog, mitochondrial protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii PY03776 Protein (E. coli)
VAng-Wyb7982-inquire inquire Ask for price
Description: Plasmodium Yoelii tRNA-splicing ligase RtcB homolog protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii PY05061 Protein (E. coli)
VAng-Wyb7986-inquire inquire Ask for price
Description: Plasmodium Yoelii Putative chloroquine resistance transporter protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii PY05764 Protein (E. coli)
VAng-Wyb7990-inquire inquire Ask for price
Description: Plasmodium Yoelii Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial protein, partial, recombinant protein.

Recombinant Plasmodium Yoelii PY07549 Protein (E. coli)
VAng-Wyb7998-inquire inquire Ask for price
Description: Plasmodium Yoelii Pescadillo homolog protein, partial, recombinant protein.

Recombinant Plasmodium Falciparum GGI.R1 Protein (E. coli)
VAng-Wyb6447-inquire inquire Ask for price
Description: Plasmodium Falciparum Glucose-6-phosphate isomerase, partial, recombinant protein.

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit
BS467 10Preps
EUR 78.28

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit
BS468 50Preps
EUR 133.08

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit
BS469 250preps
EUR 364.32

Recombinant Plasmodium Falciparum GARP Protein (E. coli)
VAng-Wyb6439-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate FC27 / Papua New Guinea) Glutamic acid-rich protein, partial, recombinant protein.

Recombinant Plasmodium Falciparum RPII Protein (E. coli)
VAng-Wyb6796-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate CDC / Honduras) DNA-directed RNA polymerase II subunit RPB1, partial, recombinant protein.

Recombinant Plasmodium Falciparum rpoB Protein (E. coli)
VAng-Wyb6809-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) DNA-directed RNA polymerase subunit beta, partial, recombinant protein.

Recombinant Plasmodium Falciparum S230 Protein (E. coli)
VAng-Wyb6826-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate NF54) Transmission-blocking target antigen S230, partial, recombinant protein.

Recombinant Plasmodium Falciparum AMA1 Protein (E. coli)
VAng-Wyb6353-500g 500 µg
EUR 5282.4
Description: Plasmodium Falciparum AMA1, recombinant protein.

96-Well Plate Plasmid DNA Miniprep Kit
BS415 5XPlates, 480prep
EUR 300.37

96-Well Plate Plasmid DNA Miniprep Kit
BS4152 2XPlates, 192prep
EUR 186.19

Recombinant Plasmodium Berghei PB000816.02.0 Protein (E. coli)
VAng-Wyb7659-inquire inquire Ask for price
Description: Plasmodium Berghei (strain Anka) tRNA-splicing ligase RtcB homolog protein, partial, recombinant protein.

Recombinant Plasmodium Falciparum HSP70 Protein (E. coli)
VAng-Wyb6502-inquire inquire Ask for price
Description: Plasmodium Falciparum Heat shock 70 kDa protein, partial, recombinant protein.

Recombinant Plasmodium Falciparum POLD1 Protein (E. coli)
VAng-Wyb6747-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate K1 / Thailand) DNA polymerase delta catalytic subunit, partial, recombinant protein.

Recombinant Plasmodium Falciparum trxr2 Protein (E. coli)
VAng-Wyb6879-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) Thioredoxin reductase 2, partial, recombinant protein.

Recombinant Plasmodium Chabaudi PC000176.04.0 Protein (E. coli)
VAng-Wyb7735-inquire inquire Ask for price
Description: Plasmodium Chabaudi Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial protein, recombinant protein.

Recombinant Plasmodium Chabaudi PC000321.02.0 Protein (E. coli)
VAng-Wyb7739-inquire inquire Ask for price
Description: Plasmodium Chabaudi Protein SEY1 homolog, partial, recombinant protein.

Recombinant Plasmodium Falciparum PFS230 Protein (E. coli)
VAng-Wyb6733-inquire inquire Ask for price
Description: Plasmodium Falciparum (isolate 3D7) Gametocyte surface protein P230, partial, recombinant protein.

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N150 500 units
EUR 122.4

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N152 5x500 units
EUR 472.8

DFS-"HOT" Taq DNA Polymerase (DNA-free sensitive, E-coli. DNA Free)
N154 20x500 units
EUR 1830

Human HLA-E shRNA Plasmid
20-abx952140
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

96-well E. coli Topo IV DNA Cleavage Assay Kit Plus
T4C96KE 96 assays
EUR 597.7
Description: This product includes all the components in TDC96K plus 26 ul of 100 x E. coli Topo IV.

Genorise® Plasmid DNA Extraction Kit 1000
GR101073 1000
EUR 850

Genorise® Plasmid DNA Extraction Kit 100
GR101073-100 100
EUR 144

Genorise® Plasmid DNA Extraction Kit 200
GR101073-200 200
EUR 258

Genorise® Plasmid DNA Extraction Kit 50
GR101073-50 50
EUR 87

Genorise® Plasmid DNA Extraction Kit 500
GR101073-500 500
EUR 480

Escherichia coli (E. coli) Antibody (FITC)
abx411322-1ml 1 ml
EUR 610.8

Escherichia coli (E. coli) Antibody (Biotin)
abx415713-1ml 1 ml
EUR 861.6

Escherichia coli (E. coli) Antibody (Biotin)
abx411321-1ml 1 ml
EUR 710.4

Plasmid DNA Extraction Kit (Rnase A included)
PL05 5 preps Ask for price

Plasmid DNA Extraction Kit (Rnase A included)
PL100 100 preps
EUR 156

Plasmid DNA Extraction Kit (Rnase A included)
PL200 200 preps
EUR 226.8

Plasmid DNA Extraction Kit (Rnase A included)
PL50 50 preps
EUR 112.8

High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA)
20-abx098008
  • EUR 260.40
  • EUR 427.20
  • 1 ml
  • 5 ml

E. coli/Coliform Count Plate
FSA1010 PK500
EUR 822

E. coli/Coliform Count Plate
FSA1012 PK50
EUR 91.2

Genorise® Feline EGF Expression Plasmid DNA
GR233001 10 µg
EUR 199

E. coli Topo IV DNA Relaxation Assay Kit Plus (enzyme included)
T4RA-100KE 100 assays
EUR 561.75
Description: This product includes 480 ul of 10 x Buffer, 405 ul of 10 x supercoiled plasmid DNA, 45 ul of 100 x E. coli topoisomerase IV, 420 ul of 10 mM ATP and 20 ul of 1500 x fluorescence dye H19 for 100 assays of DNA relaxation reactions in a 96-well assay format.

ExoFectin Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome)
P402 - Ask for price

Plasmid DNA Extraction Mini Kit (300prep)-capless
FAPDE-300-CAPLESS 300 preps
EUR 206.4

Genorise® Feline TNFA Expression Plasmid DNA
GR233004 10 µg
EUR 199

Plasmid DNA Midi Endotoxin Removal kit(25prep)-spin column
FAPDE-005B-025 25 preps
EUR 198

Plasmid DNA Midi Endotoxin Removal kit(50prep)-spin column
FAPDE-005B-050 50 preps
EUR 272.4

Plasmid DNA Maxi Endotoxin Removal kit(10prep)-spin column
FAPDE-005C-010 10 preps
EUR 186

Plasmid DNA Maxi Endotoxin Removal kit(20prep)-spin column
FAPDE-005C-020 20 preps
EUR 249.6

Genorise® Pure Plasmid DNA Mini Kit 50
GR101078 50
EUR 149

Genorise® Pure Plasmid DNA Mini Kit 100
GR101078-100 100
EUR 267

Genorise® Pure Plasmid DNA Mini Kit 200
GR101079 200
EUR 477

Genorise® Pure Plasmid DNA Mini Kit 500
GR101079-500 500
EUR 1074

E. coli O 157
abx291002-100mg 100 mg
EUR 744

QPCR Kit DNA Escherichia coli
MOL6714 EACH
EUR 1118.9

Recombinant Plasmodium Yoelii MSP-1 Protein (E. coli)
VAng-Wyb7930-inquire inquire Ask for price
Description: Plasmodium Yoelii Merozoite surface protein 1, partial, recombinant protein.

E. coli DNA Topoisomerase I Assay Kit Plus-100 (enzyme included)
DRA100KE 100 assays
EUR 590.51
Description: This product includes 480 ul of 10 x Buffer T1, 405 ul of 10 x supercoiled plasmid DNA, 20 ul of 1500 x Dye H19, 3000 ul of 10 x H19 dilution buffer and 42 ul of 100 x E. coli topoisomerase I. It is for 100 DNA relaxation assays in a 96-well plate format or 200 assays in a 384-well assay format

E. coli DNA Topoisomerase IV Assay Kit Plus-100 (enzyme included)
DDC100KE 100 assays
EUR 681.29
Description: This product includes the 600 ul of assay buffer (Buffer T4), 520 ul of 10 x concatenated DNA, 120 ul of 50 x ATP (10 mM), 550 ul of 0.4 M EDTA, 780 ul of 20 x fluorescence dye, 100 spin columns and 55ul of 100 x E.coli topoisomerase IV (500 nM) for 100 E. coli topoisomerase IV DNA decatenation assays.

Recombinant Plasmodium Fragile AMA-1 Protein (E. coli)
VAng-Wyb7787-inquire inquire Ask for price
Description: Plasmodium Fragile Apical membrane antigen 1 protein, partial, recombinant protein.

Transposition of Bordetella pertussis transposon in E. coli chromosome has been studied on a mannequin of exclusion of donor multicopy pKK3 plasmid with coumermicin. TnBP3 induced the formation of co-integrates between the plasmid and chromosome. The construction of co-integrate was decided. Info of exclusion of built-in construction and transposon transposition inside built-in plasmid into new websites on a recipient chromosome have been detected. Relationship between these processes and exercise of bacterial cell recombination system has been decided.

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