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A two-plasmid system for identification of promoters recognized by RNA polymerase containing extracytoplasmic stress response sigma(E) in Escherichia coli.

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A two-plasmid system for identification of promoters recognized by RNA polymerase containing extracytoplasmic stress response sigma(E) in Escherichia coli.

We have now beforehand established a two-plasmid system in Escherichia coli for identification of promoters acknowledged by RNA polymerase containing a heterologous sigma issue. Makes an attempt to optimize this method for identification of promoters acknowledged by RNA polymerase containing E. coli extracytoplasmic stress response sigma(E) failed owing to excessive toxicity of the expressed rpoE. A brand new system for identification of sigma(E)-cognate promoters was established, and verified utilizing the 2 recognized sigma(E)-dependent promoters, rpoEp2 and degPp. Expression of the sigma(E)-encoding rpoE gene was underneath the management of the AraC-dependent P(BAD) promoter.

A low stage of arabinose induced a non-toxic, nevertheless, ample stage of sigma(E) to work together with the core enzyme of RNA polymerase. Such an RNA polymerase holoenzyme acknowledged each recognized sigma(E)-dependent promoters, rpoEp2 and degPp, which have been cloned within the appropriate promoter probe plasmid, upstream of a promoterless lacZ alpha reporter gene. This new system has proved to be helpful for identification of E. coli sigma(E)-cognate promoters. Furthermore, the system could possibly be used for identification of ECF sigma-cognate promoters from different micro organism.

An built-in flow-injection processing (FIP) system for the quantification of plasmids throughout cultivation is described. The system performs on-line sampling, cell lysis, and quantification of plasmids in an built-in method throughout cultivation of E. coli. The system was operated through the use of a miniaturized expanded-bed column which can be utilized for dealing with samples containing cells and cell particles with out interfering with the binding evaluation. Two varieties of detectors (one measuring UV absorbance at 254 nm and a fluorometer) are used for on-line plasmid detection. The system was developed utilizing customary options and it was efficiently utilized in monitoring plasmid contents throughout a cultivation of E. coli.

Complementation between dimeric mutants as a probe of dimer-dimer interactions in tetrameric dihydrofolate reductase encoded by R67 plasmid of E. coli.

The impact of mutations on the interactions between dimers in R67 dihydrofolate reductase (R67 DHFR), a tetrameric enzyme conferring resistance to trimethoprim, was investigated by site-directed mutagenesis mixed with phenotypic, enzymatic, and biochemical evaluation. Some 14 mutants at two positions concerned in a hydrogen bond between dimers have been constructed. All have been proven to be dimers. Nevertheless, complementation between pairs of dimeric mutated proteins resulted within the restoration of the enzymatic exercise and heterotetramer formation. A combinatorial method was set as much as create effectively such heterotetramers and establish the complementing pairs of mutations. A dozen of such pairs have been discovered.

An correct methodology was set as much as measure the affiliation of the complementing dimers in a “quasi-isologous” heterotetramer and used to review the consequences of mutations and pH on the affiliation. Thus, the pair of proteins bearing respectively the S59A and H62L mutations was proven to type heterotetramers with catalytic properties near these of the wild-type protein. Its affiliation was as robust as that of the wild-type protein at cytoplasmic pH (6. 5), and was extra steady at decrease pH values.A double-mutant protein bearing concurrently the S59A and H62L mutations was produced and analyzed.

Its affiliation was weakened by 1.2 kcal/mol as in comparison with the wild-type enzyme at pH 6.5 however was insensitive to pH. Evaluating the vitality of affiliation between dimers within the wild-type protein, the heterotetramer and the double mutant allowed us to dissect the consequences of the pH and of the molecular context on a subset of interactions between the R67 DHFR subunits.

A two-plasmid system for identification of promoters recognized by RNA polymerase containing extracytoplasmic stress response sigma(E) in Escherichia coli.

Immunoadjuvant actions of E. coli– and plasmid-expressed recombinant hen IFN-alpha/beta, IFN-gamma and IL-1beta in 1-day- and 3-week-old chickens.

Within the current examine we assessed the capability of recombinant E. coli- or plasmid-expressed hen interferons (IFN) and hen IL-1beta, to exert immunostimulatory actions for humoral immune responses, in day-old and grownup chickens. We noticed that each recombinant E. coli-expressed hen IFN-alpha/beta and IFN-gamma facilitated the induction of a major and in addition a secondary antibody response, utilizing tetanus toxoid (TT) as a bacterial mannequin antigen, in immunologically mature 3-week-old chickens. In distinction, no enchancment of antibody both kind of hen IFN was co-injected with inactivated Infectious Bursal Illness Virus (IBDV) antigen.

TT-specific antibody formation was marginally elevated by co-injection of recombinant E. coli-expressed hen IL-1beta. Mixed administration of IFN-alpha/beta plus IFN-gamma or IL-beta elevated responses to TT in an additive, however not synergistic style. Remarkably, no augmentation of antibody responses particular for TT, nor IBDV, was famous in day-old birds, receiving IFN-alpha/beta or IFN-gamma as adjuvant. Additionally, intramuscular immunization of 3-week-old birds, utilizing plasmids encoding IFN-alpha/beta along with TT protein antigen, considerably elevated the pace and magnitude of TT-specific antibody responses. Plasmids encoding hen IL-beta or IFN-gamma had a minimal or inhibitory impact, respectively. These knowledge point out a possible for hen cytokines as immunoadjuvant for explicit varieties of hen vaccine antigens.

Human PBRM1 shRNA Plasmid
20-abx960543
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  • 150 µg
  • 300 µg

Escherichia coli Plasmid-derived single-stranded DNA-binding protein (ssbF)
1-CSB-EP323579ENV
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  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Escherichia coli Plasmid-derived single-stranded DNA-binding protein(ssbF) expressed in E.coli

Recombinant Escherichia coli Plasmid-derived single-stranded DNA-binding protein(ssbF)
AP71017 1mg
EUR 2826

Recombinant Escherichia coli Plasmid-derived single-stranded DNA-binding protein (ssbF)
CSB-EP323579ENV 2869 mg Ask for price

E. coli DNA Ligase
M1217-100 each
EUR 385.2

E. coli DNA Gyrase
TG2000G-1 100 units
EUR 388.8

E. coli DNA Gyrase
TG2000G-3 500 units
EUR 846

E. coli DNA Gyrase
TG2000G-5 1000 units
EUR 1396.8

E. coli DNA Gyrase
TG2000G-7 2000 units
EUR 2203.2

ApexPrep DNA Plasmid Miniprep Column Only
A5002-300 300 tests
EUR 136
Description: used for isolation of plasmid DNA

ApexPrep DNA Plasmid Miniprep Column Only
A5002-50 50 tests
EUR 26
Description: used for isolation of plasmid DNA

T4 DNA Ligase Plasmid
PVT7008 2 ug
EUR 614.4

E. coli RecQ DNA helicase
01-003 50 ug
EUR 574.8
Description: The E. coli RecQ DNA helicase is available in Europe and for worldwide shipping via Gentaur.

E. coli RecQ DNA helicase
01-004 250? g
EUR 1858.8
Description: The E. coli RecQ DNA helicase is available in Europe and for worldwide shipping via Gentaur.

E. coli DNA Polymerase I
M1210-100 each
EUR 307.2

DNA Polymerase I (E. coli)
RK20530 2500U
EUR 109

EZ-10 Spin Column Plasmid DNA Miniprep Kit, Low Copy Plasmid
BS4139 50Preps
EUR 105.67

EZ-10 Spin Column Plasmid DNA Miniprep Kit, Low Copy Plasmid
BS4149 100preps
EUR 136.74

Relaxed plasmid DNA - 0.050 mg
RDNA-050UG 0.050 mg
EUR 181.67
Description: This product includes 0.050 mg of relaxed plasmid DNA at 1 mg/ml in TE buffer

EZ-10 Spin Column Plasmid DNA Min-preps Kit, Low Copy Plasmid
BS6149 250preps
EUR 251.05

Supercoiled plasmid DNA - 0.050 mg
SDNA-050UG 0.050 mg
EUR 160.91
Description: This product includes 0.050 mg of supercoiled plasmid DNA at 1 mg/ml in TE buffer

ApexPrep DNA Plasmid Miniprep Kit
A5001-300 300 tests
EUR 160
Description: used for isolation of plasmid DNA

ApexPrep DNA Plasmid Miniprep Kit
A5001-50 50 tests
EUR 32
Description: used for isolation of plasmid DNA

Relaxed plasmid DNA - 1 mg
RDNA-1MG 1 mg
EUR 2298.23
Description: This product includes 1 mg of relaxed plasmid DNA at 1 mg/ml in TE buffer

Plasmid DNA Maxiprep (10 preps)
9K-006-0023 10Preps
EUR 257.32

Plasmid DNA Maxiprep (30 preps)
9K-006-0026 30preps
EUR 651.95

Supercoiled Plasmid DNA -1 mg
SDNA-1MG 1 mg
EUR 1752.66
Description: This product includes 1 mg of supercoiled plasmid DNA at 1 mg/ml concentration in TE buffer.

E. coli O157:H7 Genomic DNA
MBT102-250R 1 unit
EUR 119.62
Description: E. coli O157:H7 Genomic DNA

Plasmid DNA Rapidprep Mini Kit
TBS6011 200 samples
EUR 190

E. coli DNA polymerase Assay Kit Plus (E. coli DNA pol III alpha subunit included)
DPA100KE 100 assays
EUR 444.91
Description: This product includes all the reagents in the DNA Polymerase III Alpha Assay Kit (Catalog No. DPA100K) plus 35 ul of 100 x E. coli DNA polymerase III alpha subunit.

Large Plasmid DNA Extraction Kit
PDL004 each
EUR 3

Large Plasmid DNA Extraction Kit
PDL100 each
EUR 43.5

Large Plasmid DNA Extraction Kit
PDL300 each
EUR 117.5

EZ-10 Spin Column Plasmid DNA Miniprep Kit
BS413 50Preps
EUR 91.97

EZ-10 Spin Column Plasmid DNA Miniprep Kit
BS414 100preps
EUR 114.82

Plasmid DNA Isolation Solution I
10760028-1 250 mL
EUR 29.83

Plasmid DNA Isolation Solution I
10760028-2 500 mL
EUR 45.3

Plasmid DNA Isolation Solution II
10760029-1 250 mL
EUR 29.83

Plasmid DNA Isolation Solution II
10760029-2 500 mL
EUR 37.89

Deoxybrevianamide E
D083-1MG 1 mg
EUR 187.71
Description: C21H25N3O2

Deoxybrevianamide E
D083-5MG 5 mg
EUR 656.34
Description: C21H25N3O2

Berkeleylactone E
B087-1MG 1 mg
EUR 142.63
Description: C20H32O7  

Berkeleylactone E
B087-5MG 5 mg
EUR 498.97
Description: C20H32O7  

Chevalone E
C257-0.5MG 0.5 mg
EUR 150.43
Description: C20H24O4  

Chevalone E
C257-2.5MG 2.5 mg
EUR 525.85
Description: C20H24O4  

Cytochalasin E
C197-1MG 1 mg
EUR 77.6
Description: C28H33NO7

Cytochalasin E
C197-5MG 5 mg
EUR 270.51
Description: C28H33NO7

Oligomycin E
O021-1MG 1 mg
EUR 207.65
Description: C45H72O13

Oligomycin E
O021-5MG 5 mg
EUR 726.13
Description: C45H72O13

Paraherquamide E
P070-0.5MG 0.5 mg
EUR 265.74
Description: C28H35N3O4

Paraherquamide E
P070-2.5MG 2.5 mg
EUR 928.58
Description: C28H35N3O4

Phenelfamycin E
P078-1MG 1 mg
EUR 207.65
Description: C65H95NO21

Phenelfamycin E
P078-5MG 5 mg
EUR 726.13
Description: C65H95NO21

Roquefortine E
R035-1MG 1 mg
EUR 146.96
Description: C27H31N5O2

Roquefortine E
R035-5MG 5 mg
EUR 513.71
Description: C27H31N5O2

Plasmid DNA Isolation Solution III
10760030-1 250 mL
EUR 28.07

Plasmid DNA Isolation Solution III
10760030-2 500 mL
EUR 43

EZ-10 Spin Column Plasmid DNA Min-preps Kit
BS614 250preps
EUR 199.9

Plasmid DNA Extraction and Purification
EP10014 100 Tests
EUR 403.2

E. coli DNA Primase Assay Kit Plus (E. coli primase-helicase included)
EGA100KE 100 assays
EUR 444.91
Description: This product includes all the kit components in E. coli DNA Primase Assay Kit (Catalog No. EGA100K ) plus 45 ul of 100 x E. coli primase-helicase complex. It is for 100 assays of E.coli DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format.

EZ-500 Spin Column Plasmid DNA Maxi-preps Kit
BS4654 4preps
EUR 150.92

EZ-500 Spin Column Plasmid DNA Maxi-preps Kit
BS466 20Preps
EUR 466.63

QC Buffer for Plasmid DNA Isolation
10760036-1 250 mL
EUR 25.45

QC Buffer for Plasmid DNA Isolation
10760036-2 500 mL
EUR 43.01

QF Buffer for Plasmid DNA Isolation
10760037-1 250 mL
EUR 24.44

QF Buffer for Plasmid DNA Isolation
10760037-2 500 mL
EUR 43.01

QF Buffer for Plasmid DNA Isolation
10760037-3 1 L
EUR 76.87

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit
BS467 10Preps
EUR 78.28

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit
BS468 50Preps
EUR 133.08

EZ-10 Spin Column Yeast Plasmid DNA Miniprep Kit
BS469 250preps
EUR 364.32

QBT Buffer for Plasmid DNA Isolation
10760035-1 250 mL
EUR 34.22

QBT Buffer for Plasmid DNA Isolation
10760035-2 500 mL
EUR 49.13

QBT Buffer for Plasmid DNA Isolation
10760035-3 1 L
EUR 71.93

Plasmid DNA Extraction Mini Kit (100prep)
APPDE_100 100 extractions
EUR 20

Plasmid DNA Extraction Mini Kit (300prep)
APPDE_300 300 extractions
EUR 50

Plasmid DNA Extraction Mini Kit (100prep)
FAPDE-001 100 preps
EUR 168

Plasmid DNA Extraction Mini Kit (300prep)
FAPDE-001-1 300 preps
EUR 241.2

Plasmid DNA Extraction Mini Kit (100prep)
FAPDE-100 100 preps
EUR 154.8

Plasmid DNA Extraction Mini Kit (300prep)
FAPDE-300 300 preps
EUR 206.4

Plasmid DNA Extraction Mini Kit (100prep)
FAPDE_001 1 kit
EUR 31.5

Plasmid DNA Extraction Mini Kit (300prep)
FAPDE_001-1 1 kit
EUR 106.5

Plasmid DNA Extraction Mini Kit (100prep)
FAPDE_100 1 kit
EUR 22.5

Plasmid DNA Extraction Mini Kit (300prep)
FAPDE_300 1 kit
EUR 78.5

E. coli DNA ligase - for 100 assays
LIG-100EC 100 assays
EUR 309.4
Description: This product includes 10 ul of 500 nM E. coli ligase.

E. coli DNA gyrase - for 100 assays
TOP2-100EC 100 assays
EUR 309.4
Description: This product includes wild-type E. coli gyrase for 100 assays (40 ul of 2000 nM stock solution)

Presto™ Plasmid DNA Concentration Kit
PC0002 each
EUR 3

Presto™ Plasmid DNA Concentration Kit
PC0250 each
EUR 40

Presto™ Plasmid DNA Concentration Kit
PC0251 each
EUR 30

Presto™ Plasmid DNA Concentration Kit
PC0500 each
EUR 72.6

Presto™ Plasmid DNA Concentration Kit
PC0501 each
EUR 53.5

Presto™ Plasmid DNA Concentration Kit
PC1000 each
EUR 128

Presto™ Plasmid DNA Concentration Kit
PC1001 each
EUR 87

E. coli DNA primase - for 100 assays
DNAG-100EC 100 assays
EUR 309.4
Description: This product includes 45 ul of 10 uM E. coli primase-helicase complex

E. coli DNA Topoisomerase I Assay Kit Plus (E. coli Topo I included)
DRA020KE 20 assays
EUR 221.78
Description: This product includes 160 ul of 10 x Buffer T1, 84 ul of 10 x supercoiled plasmid DNA, 10 ul of 100 x E. coli topoisomerase I, 8 ul of 1500 x Dye H19 and 500 ul of 10 x H19 dilution buffer. It is for 20 DNA relaxation assays in a 96-well plate format or 40 assays in a 384-well assay format.

E. coli DNA Topoisomerase IV Assay Kit Plus (E. coli Topo IV included)
DDC020KE 20 assays
EUR 221.78
Description: This product includes all the components in DNA Topoisomerase IV Assay Kit (Catalog No. DDC020K)plus 12ul of 100 x E.coli topoisomerase IV (500 nM) for 20 E. coli topoisomerase IV DNA decatenation assays.

Desmethyl PBR28
D292445 10mg
EUR 719
Description: 1005325-42-7

E. coli DNA helicase - for 100 assays
DNAB-100EC 100 assays
EUR 309.4
Description: This product includes 33 ul of 20 uM E. coli DNA helicase.

T4 DNA Ligase, expressed in E. coli
GZ9900-1 1
EUR 150.4

T4 DNA Ligase, expressed in E. coli
GZ9900-1KIU 1 KIU
EUR 217.2

T4 DNA Ligase, expressed in E. coli
GZ9900-5 5
EUR 435.1

T4 DNA Ligase, expressed in E. coli
GZ9900-5KIU 5 KIU
EUR 562.8

96-well plate Plasmid DNA Prep (mini)
TBS6012 2x 96-well Plates
EUR 442

Double Stranded DNA (dsDNA) plasmid Peptide
GWB-T00017 1mg Ask for price

E. coli NAD+-dependent DNA ligase assay kit Plus (E. coli ligase included)
NLA100KE 100 assays
EUR 444.91
Description: This product includes 800 ul of 10 x buffer, 44 ul of 100x DNA substrate, 44 ul of 100 x NAD+, 21 ml of Reagent T, 10 ul of 500 x E. coli ligase and 220 ul of 10 x fluorescence dye for 100 assays.

HiPurA® Plasmid DNA Miniprep Purificatio
MB508-20PR 1 unit
EUR 22.08
Description: HiPurA® Plasmid DNA Miniprep Purificatio

HiPurA® Plasmid DNA Miniprep Purificatio
MB508-50PR 1 unit
EUR 46.8
Description: HiPurA® Plasmid DNA Miniprep Purificatio

HiPurA® Plasmid DNA Midiprep Purificatio
MB509-10PR 1 unit
EUR 80.49
Description: HiPurA® Plasmid DNA Midiprep Purificatio

HiPurA® Plasmid DNA Midiprep Purificatio
MB509-25PR 1 unit
EUR 191.86
Description: HiPurA® Plasmid DNA Midiprep Purificatio

HiPurA® Plasmid DNA Maxiprep Purificatio
MB510-10PR 1 unit
EUR 125.09
Description: HiPurA® Plasmid DNA Maxiprep Purificatio

HiPurA® Plasmid DNA Maxiprep Purificatio
MB510-25PR 1 unit
EUR 303.42
Description: HiPurA® Plasmid DNA Maxiprep Purificatio

HLA-E cloning plasmid
CSB-CL320269HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the HLA-E gene.

E. coli DNA Topoisomerase II (Gyrase) Assay Kit Plus (E. coli gyrase included)
DSA020KE 20 assays
EUR 221.78
Description: This product includes160 ul of 10 x Buffer T2, 84 ul of 10 x relaxed DNA, 6 ul of 1500 x H19 dye, 90 ul of 10 x ATP and 550 ul of 10 x H19 dilution buffer. It is for 20 assays in 96-well plate format. E. coli gyrase is not included.

Plasmid DNA extraction and purification kit
K1445-100 each
EUR 444

Plasmid DNA Midi Endotoxin Removal kit(25prep)-spin column
FAPDE-005B-025 25 preps
EUR 198

Plasmid DNA Midi Endotoxin Removal kit(50prep)-spin column
FAPDE-005B-050 50 preps
EUR 272.4

Plasmid DNA Maxi Endotoxin Removal kit(10prep)-spin column
FAPDE-005C-010 10 preps
EUR 186

Plasmid DNA Maxi Endotoxin Removal kit(20prep)-spin column
FAPDE-005C-020 20 preps
EUR 249.6

96-Well Plate Plasmid DNA Miniprep Kit
BS415 5XPlates, 480prep
EUR 300.37

96-Well Plate Plasmid DNA Miniprep Kit
BS4152 2XPlates, 192prep
EUR 186.19

pDONR223-HLA-E Plasmid
PVTB00773 2 ug
EUR 427.2

Recombinant Plasmodium Falciparum DNA-directed RNA polymerase III subunit RPC1 Protein (E. coli)
VAng-Wyb6412-inquire inquire Ask for price
Description: Plasmodium Falciparum DNA-directed RNA polymerase III subunit RPC1, partial, recombinant protein.

Plasmid DNA Isolation Solutions I, II, III
10760027-1 1 Kit
EUR 85.98

Genorise® Plasmid DNA Extraction Kit 1000
GR101073 1000
EUR 850

Genorise® Plasmid DNA Extraction Kit 100
GR101073-100 100
EUR 144

Genorise® Plasmid DNA Extraction Kit 200
GR101073-200 200
EUR 258

Genorise® Plasmid DNA Extraction Kit 50
GR101073-50 50
EUR 87

Genorise® Plasmid DNA Extraction Kit 500
GR101073-500 500
EUR 480

HiPurA® 96 Plasmid DNA Purification Kit
MB518-1X96PR 1 unit
EUR 153.64
Description: HiPurA® 96 Plasmid DNA Purification Kit

HiPer® Plasmid DNA Cloning Teaching Kit
HTBM022E-5PR 1 unit
EUR 83.75
Description: HiPer® Plasmid DNA Cloning Teaching Kit

Uracil DNA Glycosylase Modified, from E. coli
GZ6540-5 5
EUR 1067.8

Uracil DNA Glycosylase Modified, from E. coli
GZ6540-500 500
EUR 237.2

Uracil DNA Glycosylase Modified, from E. coli
GZ6540-500IU 500 IU
EUR 322.8

Uracil DNA Glycosylase Modified, from E. coli
GZ6540-5KIU 5 KIU
EUR 1328.4

E. coli Adenine DNA Glycosylase (MutY) Protein
abx074009-96tests 96 tests
EUR 225

HiPurA® Endotoxin free Plasmid DNA Minip
MB513-20PR 1 unit
EUR 55.28
Description: HiPurA® Endotoxin free Plasmid DNA Minip

HiPurA® Endotoxin free Plasmid DNA Minip
MB513-50PR 1 unit
EUR 126.42
Description: HiPurA® Endotoxin free Plasmid DNA Minip

HiPurA® Endotoxin free Plasmid DNA Midip
MB514-10PR 1 unit
EUR 68.13
Description: HiPurA® Endotoxin free Plasmid DNA Midip

HiPurA® Endotoxin free Plasmid DNA Midip
MB514-25PR 1 unit
EUR 145.78
Description: HiPurA® Endotoxin free Plasmid DNA Midip

HiPurA® Endotoxin free Plasmid DNA Maxip
MB515-10PR 1 unit
EUR 188.4
Description: HiPurA® Endotoxin free Plasmid DNA Maxip

HiPurA® Endotoxin free Plasmid DNA Maxip
MB515-25PR 1 unit
EUR 470.14
Description: HiPurA® Endotoxin free Plasmid DNA Maxip

Azithromycin impurity E EvoPure®
A074-5MG 5 mg
EUR 593.04
Description: C36H68N2O12

HiPurA® Yeast Plasmid DNA Purification K
MB550-20PR 1 unit
EUR 41.87
Description: HiPurA® Yeast Plasmid DNA Purification K

HiPurA® Yeast Plasmid DNA Purification K
MB550-250PR 1 unit
EUR 415.6
Description: HiPurA® Yeast Plasmid DNA Purification K

HiPurA® Yeast Plasmid DNA Purification K
MB550-50PR 1 unit
EUR 99.65
Description: HiPurA® Yeast Plasmid DNA Purification K

HiPer® Plasmid DNA Extraction Teaching K
HTBM002E-10PR 1 unit
EUR 38.51
Description: HiPer® Plasmid DNA Extraction Teaching K

HiPer® Plasmid DNA Extraction Teaching K
HTBM002E-20PR 1 unit
EUR 55.13
Description: HiPer® Plasmid DNA Extraction Teaching K

HiPer® Plasmid DNA Extraction Teaching K
HTBM003E-20PR 1 unit
EUR 65.04
Description: HiPer® Plasmid DNA Extraction Teaching K

Plasmid DNA Extraction Kit (Rnase A included)
PL05 5 preps Ask for price

Plasmid DNA Extraction Kit (Rnase A included)
PL100 100 preps
EUR 156

Plasmid DNA Extraction Kit (Rnase A included)
PL200 200 preps
EUR 226.8

Plasmid DNA Extraction Kit (Rnase A included)
PL50 50 preps
EUR 112.8

Plasmid DNA Extraction Kit (Rnase A included)
PL96-05 5x96 preps
EUR 595

Genorise® Pure Plasmid DNA Mini Kit 50
GR101078 50
EUR 149

Genorise® Pure Plasmid DNA Mini Kit 100
GR101078-100 100
EUR 267

Genorise® Pure Plasmid DNA Mini Kit 200
GR101079 200
EUR 477

Genorise® Pure Plasmid DNA Mini Kit 500
GR101079-500 500
EUR 1074

Genorise® Feline EGF Expression Plasmid DNA
GR233001 10 µg
EUR 199

EZ-10 Spin Column Endotoxin-Free Plasmid DNA Miniprep Kit
BS71918 50Preps
EUR 149.52

Plasmid DNA Extraction Mini Kit (300prep)-capless
FAPDE-300-CAPLESS 300 preps
EUR 206.4

Genorise® Feline TNFA Expression Plasmid DNA
GR233004 10 µg
EUR 199

High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA)
20-abx098008
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  • 1 ml
  • 5 ml

High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA)
abx098008-100l 100 µl
EUR 262.5

High Fidelity (HiFi) PCR SuperMix (-dye) (lambda DNA, cDNA and plasmid DNA)
abx098008-200l 200 µl
EUR 475

96-well Plasmid DNA Extraction Kit (1 plate)
FAPWE-96001 1 prep
EUR 175.2

96-well Plasmid DNA Extration Kit (4 plates)
FAPWE-96004 4 preps
EUR 328.8

96-well Plasmid DNA Extraction Kit (1 plate)
FAPWE_96001 1 kit
EUR 36

Transposition of Bordetella pertussis transposon in E. coli chromosome has been studied on a mannequin of exclusion of donor multicopy pKK3 plasmid with coumermicin. TnBP3 induced the formation of co-integrates between the plasmid and chromosome. The construction of co-integrate was decided. Info of exclusion of built-in construction and transposon transposition inside built-in plasmid into new websites on a recipient chromosome have been detected. Relationship between these processes and exercise of bacterial cell recombination system has been decided.

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